Align 4-aminobutyrate transaminase subunit (EC 2.6.1.19) (characterized)
to candidate GFF179 PS417_00900 4-aminobutyrate aminotransferase
Query= metacyc::MONOMER-11537 (425 letters) >FitnessBrowser__WCS417:GFF179 Length = 425 Score = 810 bits (2091), Expect = 0.0 Identities = 409/425 (96%), Positives = 419/425 (98%) Query: 1 MSKTNASLMKRREAAVPRGVGQIHPIFADSAKNATVTDVEGREFIDFAGGIAVLNTGHVH 60 MSKTNASLMKRREAAVPRGVGQIHPIFA+SAKNATVTDVEGREFIDFAGGIAVLNTGHVH Sbjct: 1 MSKTNASLMKRREAAVPRGVGQIHPIFAESAKNATVTDVEGREFIDFAGGIAVLNTGHVH 60 Query: 61 PKIIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAV 120 PKIIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAV Sbjct: 61 PKIIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAV 120 Query: 121 KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS 180 KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGG+FRAL+PNELHGVS Sbjct: 121 KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGVFRALFPNELHGVS 180 Query: 181 IDDSIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD 240 DD+IASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPK FMKRLR LCD+HGILLIAD Sbjct: 181 DDDAIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKSFMKRLRELCDKHGILLIAD 240 Query: 241 EVQTGAGRTGTFFAMEQMGVTADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA 300 EVQTGAGRTGTFFAMEQMGV ADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA Sbjct: 241 EVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA 300 Query: 301 GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL 360 GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL Sbjct: 301 GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL 360 Query: 361 FENGDSHKPNAAAVAQVVAKARDKGLILLSCGTYGNVLRVLVPLTSPDAQLDKGLAIIEE 420 F++GD+HKPNAAAVA VVAKAR+KGLILLSCGTYGNVLRVLVPLTSPD QLDKGLAIIEE Sbjct: 361 FDDGDTHKPNAAAVASVVAKAREKGLILLSCGTYGNVLRVLVPLTSPDEQLDKGLAIIEE 420 Query: 421 CFAEL 425 CF+EL Sbjct: 421 CFSEL 425 Lambda K H 0.320 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 820 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 425 Length of database: 425 Length adjustment: 32 Effective length of query: 393 Effective length of database: 393 Effective search space: 154449 Effective search space used: 154449 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate GFF179 PS417_00900 (4-aminobutyrate aminotransferase)
to HMM TIGR00700 (gabT: 4-aminobutyrate transaminase (EC 2.6.1.19))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00700.hmm # target sequence database: /tmp/gapView.30806.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00700 [M=420] Accession: TIGR00700 Description: GABAtrnsam: 4-aminobutyrate transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.2e-168 544.2 6.6 9.3e-168 544.1 6.6 1.0 1 lcl|FitnessBrowser__WCS417:GFF179 PS417_00900 4-aminobutyrate amin Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF179 PS417_00900 4-aminobutyrate aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 544.1 6.6 9.3e-168 9.3e-168 1 418 [. 10 422 .. 10 424 .. 0.99 Alignments for each domain: == domain 1 score: 544.1 bits; conditional E-value: 9.3e-168 TIGR00700 1 qrraaavskGvgvtlrvlaakaegaelkdvdGnrlidlaagiavlnvGhshPkvveavkrqveelthtafqvvpye 76 +rr+aav++Gvg +++a +a++a++ dv+G+++id+a+giavln+Gh hPk+++av +q+++ltht+fqv+ ye lcl|FitnessBrowser__WCS417:GFF179 10 KRREAAVPRGVGQIHPIFAESAKNATVTDVEGREFIDFAGGIAVLNTGHVHPKIIAAVTEQLNKLTHTCFQVLAYE 85 69************************************************************************** PP TIGR00700 77 syvelaeklnaiaPgsgekkavllnsGaeavenavkiarkytgrpgvvafsrgfhGrtnltmaltakvkPykiGfG 152 yvel ek+na Pg kk++l+++G+eavenavkiar+ tgr gv+af++++hGrt +t+ lt+kv Py G+G lcl|FitnessBrowser__WCS417:GFF179 86 PYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAVKIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMG 161 **************************************************************************** PP TIGR00700 153 PfapevyraPlpydyrdialdkqeslddelaaiealfvadveaeqvaavvlePvqGeGGfivpakelvaavaslck 228 +++ v+ra +p ++++ + dd +a+ie++f+ d e++++aa+++ePvqGeGGf v++k +++ +++lc+ lcl|FitnessBrowser__WCS417:GFF179 162 LMPGGVFRALFPNELHGVSD------DDAIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKSFMKRLRELCD 231 ****************9887......78899********************************************* PP TIGR00700 229 ehgivliadevqtGfartGklfaieheddkPdlitvaksladGlPlsgvvGraeildapapGglGGtyaGnPlava 304 +hgi+liadevqtG rtG++fa+e +++ dl t aks+a+G+Pl+gv G+ae +da apGglGGtyaG+P+a+a lcl|FitnessBrowser__WCS417:GFF179 232 KHGILLIADEVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYAGSPIACA 307 **************************************************************************** PP TIGR00700 305 aalavldiieeeglieraeqigklvkdklielkeevpaigdvrglGamiavelv.dpdttePdaalaekiaaaala 379 aalav+++ eee l +r++ +g+++ l ++++ p+ig+vr lGamiavel d dt++P+aa + + a+a++ lcl|FitnessBrowser__WCS417:GFF179 308 AALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVELFdDGDTHKPNAAAVASVVAKARE 383 ****************************************************99789******************* PP TIGR00700 380 aGlllltaGifGniirlltPltisdelldeglkileaal 418 +Gl+ll++G +Gn++r+l Plt +de+ld+gl i+e+ + lcl|FitnessBrowser__WCS417:GFF179 384 KGLILLSCGTYGNVLRVLVPLTSPDEQLDKGLAIIEECF 422 ***********************************9876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (420 nodes) Target sequences: 1 (425 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.38 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory