Align 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; L-KDR 4-dehydrogenase; EC 1.1.1.401 (characterized)
to candidate GFF4186 PS417_21440 3-ketoacyl-ACP reductase
Query= SwissProt::Q1NEI6 (249 letters) >FitnessBrowser__WCS417:GFF4186 Length = 247 Score = 131 bits (330), Expect = 1e-35 Identities = 77/245 (31%), Positives = 138/245 (56%), Gaps = 15/245 (6%) Query: 10 GRCAIVTGGASGLGKQVAARIIAEGGAVALWDLNGDALAATQAEIDATHV---------V 60 G+ A+VTG + G+G+ +A + +G V G A +A+ AE A + + Sbjct: 5 GKVALVTGASRGIGQAIALELGRQGAVVI-----GTATSASGAERIAATLKENGVQGTGL 59 Query: 61 ALDVSDHAAVAAAAKDSAAALGKVDILICSAGITGATVPVWEFPVDSFQRVIDINLNGLF 120 L+V+ +VAA + A G IL+ +AGIT + + D + V+D NLN LF Sbjct: 60 ELNVTSDESVAAVLAEITAQFGAPAILVNNAGITRDNL-MMRMKDDEWYDVVDTNLNSLF 118 Query: 121 YCNREVVPFMLENGYGRIVNLASVAGKEGNPNASAYSASKAGVIGFTKSLGKELAGKGVI 180 ++ V+ M + +GRI+N+ SV G GN Y+++KAG+ GF+++L +E+ + + Sbjct: 119 RLSKGVLRGMTKARWGRIINIGSVVGAMGNAGQVNYASAKAGLEGFSRALAREVGSRSIT 178 Query: 181 ANALTPATFESPILDQLPQSQVDYMRSKIPMGRLGLVEESAAMVCFMASEECSFTTASTF 240 N++ P ++ + +LP++Q + + ++IP+GRLG +E A +V F+AS+ ++ T +T Sbjct: 179 VNSVAPGFIDTDMTRELPEAQREALLTQIPLGRLGQAQEIANVVTFLASDGAAYVTGATI 238 Query: 241 DTSGG 245 +GG Sbjct: 239 PVNGG 243 Lambda K H 0.318 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 158 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 249 Length of database: 247 Length adjustment: 24 Effective length of query: 225 Effective length of database: 223 Effective search space: 50175 Effective search space used: 50175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory