Alignments for a candidate for rbsA in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for rbsA in Pseudomonas simiae WCS417

Align ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 (characterized)
to candidate GFF2673 PS417_13635 D-ribose transporter ATP-binding protein

Query= CharProtDB::CH_003578
         (501 letters)



>FitnessBrowser__WCS417:GFF2673
          Length = 510

 Score =  402 bits (1034), Expect = e-116
 Identities = 221/501 (44%), Positives = 326/501 (65%), Gaps = 9/501 (1%)

Query: 1   MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKSTMMKVLTGIYTRDAGT 60
           +E LL+++GI K F G++ L    L VYPG + AL+GENGAGKST+MK+L+G Y  D G 
Sbjct: 3   LEPLLEMQGISKTFNGLRVLKTVGLKVYPGEIHALMGENGAGKSTLMKILSGAYQADPGG 62

Query: 61  LLWLGKETTFT-GPKSSQEAGIGIIHQELNLIPQLTIAENIFLGREFVNRFGKIDWKTMY 119
            + +  +   T  P +++  GI +I+QEL+L P L++AENI+LGRE + R   ID K M 
Sbjct: 63  EIRIAGQLIPTFDPATAKALGIAVIYQELSLCPNLSVAENIYLGRE-LRRGWTIDRKGME 121

Query: 120 AEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKVIIMDEPTDALTDTETES 179
           A   ++L +L   F     V  LSI ++Q+VEIA+ L   +K+++MDEPT  L+  ET+ 
Sbjct: 122 AGCIEVLQRLGAEFTPATRVSSLSIAERQLVEIARALHAHAKILVMDEPTTPLSSRETDR 181

Query: 180 LFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQFIAEREVASLTEDSLIEMMVG 239
           LF +I++L+SQG  I+YISHRM EI+ + D V+V RDG +I E    +L+ ++L++MMVG
Sbjct: 182 LFALIKQLRSQGLAIIYISHRMAEIYALSDRVSVLRDGHYIGELTRDALSAEALVKMMVG 241

Query: 240 RKLEDQYP--HLDKAPGDIRLKVDNLC-GPGVNDVSFTLRKGEILGVSGLMGAGRTELMK 296
           R L   Y   H    PG++ ++V ++  G  V   SF L  GE+LG++GL+GAGRTEL +
Sbjct: 242 RDLSGFYKKEHAAYNPGNVVMRVRDMADGKRVRHCSFDLHAGEVLGIAGLVGAGRTELAR 301

Query: 297 VLYGALPRTSGYVTLDGHEVVT-RSPQDGLANGIVYISEDRKRDGLVLGMSVKENMSLTA 355
           +++ A PRTSG + + G  V   R+P D +  G+VY++EDRK  GL L MSV +N+++ A
Sbjct: 302 LIFAADPRTSGTLEVVGKAVTPLRTPADAIRAGVVYLTEDRKAQGLFLDMSVADNINVCA 361

Query: 356 LRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRPK 415
               + AGG L      Q  +D I+  +++  S +   G LSGGNQQKV +AR L  +P 
Sbjct: 362 CVPDAHAGGVLDRDHALQRSNDAIKSLSIRVASGKVNAGALSGGNQQKVLLARLLEVKPH 421

Query: 416 VLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLSG 475
           VLILDEPTRGVD+G+K EIY++INQ    G+ I+++SSE+PE++G  DR+++M EG L  
Sbjct: 422 VLILDEPTRGVDIGSKSEIYRIINQLAQAGIGIVVISSELPEIIGTCDRVLIMREGQLVA 481

Query: 476 EF---TREQATQEVLMAAAVG 493
           E    + +  +QE ++  A G
Sbjct: 482 EVGGASGQAISQERIIDLATG 502



 Score =  100 bits (249), Expect = 1e-25
 Identities = 73/225 (32%), Positives = 109/225 (48%), Gaps = 8/225 (3%)

Query: 271 VSFTLRKGEILGVSGLMGAGRTELMKVLYGALPRT-SGYVTLDGHEVVTRSPQDGLANGI 329
           V   +  GEI  + G  GAG++ LMK+L GA      G + + G  + T  P    A GI
Sbjct: 25  VGLKVYPGEIHALMGENGAGKSTLMKILSGAYQADPGGEIRIAGQLIPTFDPATAKALGI 84

Query: 330 VYISEDRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSM 389
             I ++     L   +SV EN+ L   R   R     +   E   +    RL    TP+ 
Sbjct: 85  AVIYQELS---LCPNLSVAENIYLG--RELRRGWTIDRKGMEAGCIEVLQRLGAEFTPAT 139

Query: 390 EQAIGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSII 449
              +  LS   +Q V IAR L    K+L++DEPT  +       ++ LI Q ++ GL+II
Sbjct: 140 R--VSSLSIAERQLVEIARALHAHAKILVMDEPTTPLSSRETDRLFALIKQLRSQGLAII 197

Query: 450 LVSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGK 494
            +S  M E+  +SDR+ V+ +GH  GE TR+  + E L+   VG+
Sbjct: 198 YISHRMAEIYALSDRVSVLRDGHYIGELTRDALSAEALVKMMVGR 242


Lambda     K      H
   0.318    0.137    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 661
Number of extensions: 27
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 501
Length of database: 510
Length adjustment: 34
Effective length of query: 467
Effective length of database: 476
Effective search space:   222292
Effective search space used:   222292
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources