Align L-serine dehydratase, alpha chain; Short=SDH; EC 4.3.1.17 (characterized, see rationale)
to candidate GFF5136 PS417_26310 serine dehydratase
Query= uniprot:P33073 (292 letters) >FitnessBrowser__WCS417:GFF5136 Length = 458 Score = 138 bits (347), Expect = 3e-37 Identities = 94/284 (33%), Positives = 147/284 (51%), Gaps = 12/284 (4%) Query: 3 NTAREIIDVCNERGIKIYDLVLEEEIKNSHTTEEEIRKKLDAVIDVMHASATKNLTQSDV 62 ++A E++ +C +++ +L+L E + +EEEIR L + M + L + Sbjct: 174 SSAVELLQLCKTHNLRVAELMLANE--KTWRSEEEIRSGLMKLWRAMQDCVEQGLKHEGI 231 Query: 63 T----EYKMIDGFAKRTYEYANSGKSIVGDFLAK---AMAMAFSTSEVNASMGKIVAAPT 115 + R+ + N +++G L+ A + +E NA+ G++V APT Sbjct: 232 LPGGLNVRRRAAKLHRSLQELNK-PNVIGSTLSAMEWVNLFALAVNEENAAGGRMVTAPT 290 Query: 116 AGSSGIMPAMLVAATE-KYNFDRTTIQNGFLTSIGIGQVITKYATFAGAEGGCQAECGSA 174 G++GI+PA+L + + + L + +G + K A+ +GAE GCQ E GSA Sbjct: 291 NGAAGIIPAVLHYFMKFSEEVTEANVVDYLLGAAAVGILCKKNASISGAEVGCQGEVGSA 350 Query: 175 SAMAAAALVEMLGGTVEQALHAASITIINVLGLVCDPIAGLVQYPCTFRNASGVINAFIS 234 AMAAA L E+LG T EQ +AA I + + LGL CDP+ GLVQ PC RNA + A + Sbjct: 351 CAMAAAGLAEILGATPEQLCNAAEIGLEHNLGLTCDPVGGLVQVPCIERNAIAAVKAINA 410 Query: 235 ADLALAG-VESLVPFDEVVIAMGEVGNSMIEALRETGLGGLAGS 277 A +AL G + + D V+ M + G M + +ET GGLA S Sbjct: 411 AQMALRGDGQHFISLDRVIRTMRDTGADMHDKYKETSRGGLAVS 454 Lambda K H 0.317 0.132 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 252 Number of extensions: 11 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 292 Length of database: 458 Length adjustment: 30 Effective length of query: 262 Effective length of database: 428 Effective search space: 112136 Effective search space used: 112136 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory