Align ABC transporter for D-Trehalose, permease component 2 (characterized)
to candidate GFF3170 PS417_16225 sugar ABC transporter permease
Query= reanno::Smeli:SM_b20327 (276 letters) >FitnessBrowser__WCS417:GFF3170 Length = 266 Score = 144 bits (362), Expect = 3e-39 Identities = 86/271 (31%), Positives = 140/271 (51%), Gaps = 6/271 (2%) Query: 5 IAKRTAFYALVAVIILVAVFPFYYAILTSLKSGTALFR-IDYWPTDISLANYAGIFSHGT 63 ++KR L+ ++ L+ P Y+ + S KS T + + +P D +LANY IF+ + Sbjct: 1 MSKRKVIPLLIYILFLLV--PIYWLLNMSFKSNTEILGGLTLFPQDFTLANYKVIFTDPS 58 Query: 64 FVRNLGNSLLVATLVVAISLLLAVTAAYALARVRFRGRGLLLLTILSVSMFPQIAVLAGL 123 + NSL +L ISL +A+ AAYA +R RF G L +L+ M P L Sbjct: 59 WYTGYLNSLYYVSLNTVISLSVALPAAYAFSRYRFLGDKHLFFWLLTNRMAPPAVFLLPF 118 Query: 124 FELIRFVGIFNTPLALIFSYMIFTLPFTVWVLTTFMRDLPIEIEEAAIVDGASPWVVITR 183 F+L +G+F+T +A+ ++ +F +P VW+L FM +P EI+E A +DG S + Sbjct: 119 FQLYSSIGLFDTHIAVALAHCLFNVPLAVWILEGFMSGVPKEIDETAYIDGYSFPKFFVK 178 Query: 184 VFMPLMWPALVTTGLLAFIAAWNEFLFALTFTSSNTQRTVPVAIALLSGGSQFEIPWGNI 243 +F+PL+ + T F+ +W E L A T TS N + P+A + S I WG + Sbjct: 179 IFIPLIGSGIGVTAFFCFMFSWVELLLARTLTSVNAK---PIAAVMTRTVSASGIDWGVL 235 Query: 244 MAASVIVTVPLVVLVLIFQRRIISGLTAGGV 274 AA V+ +P ++++ + + G G V Sbjct: 236 AAAGVLTILPGMLVIWFVRNHVAKGFALGRV 266 Lambda K H 0.332 0.143 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 177 Number of extensions: 10 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 276 Length of database: 266 Length adjustment: 25 Effective length of query: 251 Effective length of database: 241 Effective search space: 60491 Effective search space used: 60491 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory