Aligments for a candidate for L-LDH in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Aligments for a candidate for L-LDH in Pseudomonas simiae WCS417

Align L-lactate dehydrogenase (cytochrome) (EC 1.1.2.3) (characterized)
to candidate GFF3053 PS417_15625 lactate dehydrogenase

Query= reanno::acidovorax_3H11:Ac3H11_1623
         (390 letters)



>FitnessBrowser__WCS417:GFF3053
          Length = 386

 Score =  582 bits (1501), Expect = e-171
 Identities = 286/383 (74%), Positives = 332/383 (86%), Gaps = 4/383 (1%)

Query: 7   ITCIEDLRAIAQRRVPRMFYDYADSGSYTEGTYRANESDFQRIKLRQRVAVNMEGRSTRT 66
           IT IEDLR +AQ+RVPRMFYDYADSGS+TE TYRANESDF  IK RQRVA N++ RS R 
Sbjct: 4   ITTIEDLRKLAQKRVPRMFYDYADSGSWTESTYRANESDFASIKFRQRVARNIDERSIRA 63

Query: 67  TMVGQDVAMPVAIAPTGLTGMQHADGEILGAKAAKAFGIPFTLSTMSICSIEDIAEHTGR 126
           TM+GQ++AMPVA+APTGL GMQHADGEIL A+AA AFG+ +TLSTMSICS+EDIAEH G+
Sbjct: 64  TMIGQEMAMPVALAPTGLAGMQHADGEILTARAAAAFGLRYTLSTMSICSLEDIAEHVGQ 123

Query: 127 HPFWFQVYVMRDRDFIERLIDRAKAANCSALQLTLDLQILGQRHKDIKNGLSAPPKPTIA 186
            PFWFQ+YVMRDR FIE+LI+RAKAA   AL LTLDLQILGQRHKD+ NGLSAPPK T+ 
Sbjct: 124 -PFWFQLYVMRDRAFIEQLIERAKAAGVDALVLTLDLQILGQRHKDLINGLSAPPKLTLP 182

Query: 187 NLINLATKPRWCLGMLGTKRRSFGNIVGHAKGVGDLSSLSSWTAEQFDPQLNWGDVEWIK 246
           N++N+ATKPRW +GMLGT+RR FGNIVGH KGV D+SSLSSWTA+QFDP+L+W DVEWIK
Sbjct: 183 NILNMATKPRWVMGMLGTQRRGFGNIVGHVKGVADMSSLSSWTAQQFDPRLSWDDVEWIK 242

Query: 247 KRWGGKLILKGIMDAEDARLAVNSGADALIVSNHGGRQLDGAPSSIAALPGIADAVAQMG 306
           K WGGKLI+KGI+D EDA LA N+GADAL+VSNHGGRQLDGAPSSI+ LP I +AV   G
Sbjct: 243 KCWGGKLIIKGILDVEDAHLAANAGADALVVSNHGGRQLDGAPSSISQLPAIVEAV---G 299

Query: 307 GGIEVWMDGGIRSGQDVLKARALGAQGTLIGRSFLYGLGAYGEAGVTRALQIIQKELDIT 366
             IEVW+DGGIRSGQDVLKA ALGA+GT+IGR  LYGLGA GEAGVT+AL+II +ELD++
Sbjct: 300 ERIEVWLDGGIRSGQDVLKAMALGAKGTMIGRPHLYGLGAMGEAGVTKALEIIARELDVS 359

Query: 367 MAFCGHTNINTVDRSILLPGTYP 389
           MA CG+ +I  V+R ILLPGT+P
Sbjct: 360 MALCGYNDIRDVNREILLPGTFP 382


Lambda     K      H
   0.320    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 519
Number of extensions: 15
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 390
Length of database: 386
Length adjustment: 30
Effective length of query: 360
Effective length of database: 356
Effective search space:   128160
Effective search space used:   128160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources