Align Aminomethyltransferase (EC 2.1.2.10) (characterized)
to candidate GFF4369 PS417_22370 glycine cleavage system protein T
Query= reanno::WCS417:GFF4369 (374 letters) >FitnessBrowser__WCS417:GFF4369 Length = 374 Score = 738 bits (1905), Expect = 0.0 Identities = 374/374 (100%), Positives = 374/374 (100%) Query: 1 MSTETLLKTPLHALHIELGARMVPFAGYDMPVQYPLGVMKEHLHTRDQAGLFDVSHMGQI 60 MSTETLLKTPLHALHIELGARMVPFAGYDMPVQYPLGVMKEHLHTRDQAGLFDVSHMGQI Sbjct: 1 MSTETLLKTPLHALHIELGARMVPFAGYDMPVQYPLGVMKEHLHTRDQAGLFDVSHMGQI 60 Query: 61 RLTGANAAKALETLVPVDILDLPVGMQRYAMFTNDQGGILDDLMVANLGNEELFLVVNAA 120 RLTGANAAKALETLVPVDILDLPVGMQRYAMFTNDQGGILDDLMVANLGNEELFLVVNAA Sbjct: 61 RLTGANAAKALETLVPVDILDLPVGMQRYAMFTNDQGGILDDLMVANLGNEELFLVVNAA 120 Query: 121 CKDQDLAHLRQHIGDQCTIEPLFEERALLALQGPAAVKVLARLAPEVTKMTFMQFATLRL 180 CKDQDLAHLRQHIGDQCTIEPLFEERALLALQGPAAVKVLARLAPEVTKMTFMQFATLRL Sbjct: 121 CKDQDLAHLRQHIGDQCTIEPLFEERALLALQGPAAVKVLARLAPEVTKMTFMQFATLRL 180 Query: 181 LGVDCYVSRSGYTGEDGFEISVPAANAESLARSLLAETEVQAIGLGARDSLRLEAGLCLY 240 LGVDCYVSRSGYTGEDGFEISVPAANAESLARSLLAETEVQAIGLGARDSLRLEAGLCLY Sbjct: 181 LGVDCYVSRSGYTGEDGFEISVPAANAESLARSLLAETEVQAIGLGARDSLRLEAGLCLY 240 Query: 241 GHDMNTDTTPIEASLLWAISKARRADGARAGGFPGADTIFTQQQAGVSRKRVGLLPQERT 300 GHDMNTDTTPIEASLLWAISKARRADGARAGGFPGADTIFTQQQAGVSRKRVGLLPQERT Sbjct: 241 GHDMNTDTTPIEASLLWAISKARRADGARAGGFPGADTIFTQQQAGVSRKRVGLLPQERT 300 Query: 301 PVREGAQIVDADGTVIGSVCSGGFGSTLGGPLAMGYLDSAFIALDTEVSALVRGKKVPLR 360 PVREGAQIVDADGTVIGSVCSGGFGSTLGGPLAMGYLDSAFIALDTEVSALVRGKKVPLR Sbjct: 301 PVREGAQIVDADGTVIGSVCSGGFGSTLGGPLAMGYLDSAFIALDTEVSALVRGKKVPLR 360 Query: 361 VSKMPFVPQRYYRG 374 VSKMPFVPQRYYRG Sbjct: 361 VSKMPFVPQRYYRG 374 Lambda K H 0.321 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 643 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 374 Length of database: 374 Length adjustment: 30 Effective length of query: 344 Effective length of database: 344 Effective search space: 118336 Effective search space used: 118336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate GFF4369 PS417_22370 (glycine cleavage system protein T)
to HMM TIGR00528 (gcvT: glycine cleavage system T protein (EC 2.1.2.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00528.hmm # target sequence database: /tmp/gapView.18283.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00528 [M=362] Accession: TIGR00528 Description: gcvT: glycine cleavage system T protein Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-107 345.2 0.0 2.3e-107 345.0 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF4369 PS417_22370 glycine cleavage sys Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF4369 PS417_22370 glycine cleavage system protein T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 345.0 0.0 2.3e-107 2.3e-107 2 361 .. 7 368 .. 6 369 .. 0.96 Alignments for each domain: == domain 1 score: 345.0 bits; conditional E-value: 2.3e-107 TIGR00528 2 krtpLydlhtelggklvdfaGwelPvqyk.slieeheavrtkaGlfDvshmgkvelsGskslkfLqrllanDvda 75 +tpL+ lh elg+++v+faG+ +Pvqy ++++eh +r++aGlfDvshmg+++l+G ++ k+L+ l++ D+ lcl|FitnessBrowser__WCS417:GFF4369 7 LKTPLHALHIELGARMVPFAGYDMPVQYPlGVMKEHLHTRDQAGLFDVSHMGQIRLTGANAAKALETLVPVDILD 81 79**************************6488******************************************* PP TIGR00528 76 LtkGkaqysvllnaqGGvvDDlivykegednrfllvvnaataekDlewlkehleeevtldtlskeisllalqGPk 150 L++G +y+ + n qGG++DDl+v + g++ ++ lvvnaa++++Dl++l++h+ ++ t++ l +e +llalqGP lcl|FitnessBrowser__WCS417:GFF4369 82 LPVGMQRYAMFTNDQGGILDDLMVANLGNE-ELFLVVNAACKDQDLAHLRQHIGDQCTIEPLFEERALLALQGPA 155 ****************************99.999***************************************** PP TIGR00528 151 aktiledlldkaveglkefffvqeaelalkkaliartGytGedGfeiavanekavelwkklveaygvkPiGLgar 225 a ++l l +v+++ + f l++ +++++r+GytGedGfei+v+ +a l+++l+++ v+ iGLgar lcl|FitnessBrowser__WCS417:GFF4369 156 AVKVLARLAP-EVTKMTFMQFA-TLRLLGVDCYVSRSGYTGEDGFEISVPAANAESLARSLLAETEVQAIGLGAR 228 *****99875.45566544444.5578899********************************************* PP TIGR00528 226 DtLrleagmaLyGqeldeeitPleaglgwvvkker.....kksdfiGravleeqkengtekklvGlemlekgiar 295 D+Lrleag++LyG++++ ++tP+ea+l w+++k r + f G +++ q++ g+ +k+vGl e+ r lcl|FitnessBrowser__WCS417:GFF4369 229 DSLRLEAGLCLYGHDMNTDTTPIEASLLWAISKARradgaRAGGFPGADTIFTQQQAGVSRKRVGLLPQERTPVR 303 *********************************99888877889******************************* PP TIGR00528 296 nelkvlltngekevGivtsGtlsPtLgknialayvdkelekiGtklevevrnklvkikvvkklfvr 361 ++ +++ ++g + +G v sG + tLg +a++y+d+ + t++ vr+k+v+++v k++fv+ lcl|FitnessBrowser__WCS417:GFF4369 304 EGAQIVDADG-TVIGSVCSGGFGSTLGGPLAMGYLDSAFIALDTEVSALVRGKKVPLRVSKMPFVP 368 **********.*****************************************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (362 nodes) Target sequences: 1 (374 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.07 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory