Align aminomethyltransferase; EC 2.1.2.10 (characterized)
to candidate GFF5324 PS417_27255 glycine cleavage system protein T
Query= CharProtDB::CH_000563 (364 letters) >FitnessBrowser__WCS417:GFF5324 Length = 360 Score = 402 bits (1032), Expect = e-116 Identities = 203/364 (55%), Positives = 265/364 (72%), Gaps = 6/364 (1%) Query: 1 MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDAGMFDVSHMTIVDLRGS 60 M Q+TPLY+ H GA+MVDF GW MPLHYGSQ++EHH VR D G+FDVSHMT++D+ G Sbjct: 1 MGQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMTVIDVTGP 60 Query: 61 RTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVYYFTEDFFRLVVNSATREK 120 + +E+L+YLLANDV +L G+ALYS MLN GGV+DD+IVY TE +RLVVN++TR++ Sbjct: 61 QAKEWLQYLLANDVERLHDCGRALYSAMLNEKGGVVDDMIVYR-TESAYRLVVNASTRDQ 119 Query: 121 DLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLFNDAQRQAVEGMKPFFGVQAG 180 D++W+ + +++ R +L+M+A+QGP+A+ K A L ++ + +KPF G G Sbjct: 120 DMTWMHAQLGNYQVQLHERPELAMLAIQGPHARQKIAELVTQSRSTLIHQLKPFEGQADG 179 Query: 181 DLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKPCGLGARDTLRLEAGMNLYGQEM 240 D FIA TGYTGE G EI LP ++A F+ LV AG+ P GLGARDTLRLEAGMNLYGQ++ Sbjct: 180 DWFIARTGYTGEDGLEIVLPADQAPGFFNDLVGAGISPIGLGARDTLRLEAGMNLYGQDI 239 Query: 241 DETISPLAANMGWTIAWEPADRDFIGREALEVQREHGTE-KLVGLVMTEKGVLRNELPVR 299 + ISPLA+NM W+IAWEPA+R+FIGR ALE ++ G + KLVGLV+ E+GVLR VR Sbjct: 240 HQDISPLASNMAWSIAWEPAERNFIGRAALEAEKATGVQFKLVGLVLEERGVLRAHQVVR 299 Query: 300 FTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGIGETAIVQIRNREMPVKVTKPVFVRN 359 N EG ITSG+FSPTL SIALARVP + A V+IR + PV+V KP FVR+ Sbjct: 300 I----ANIGEGEITSGSFSPTLSKSIALARVPTATADRAEVEIRGKWYPVRVVKPTFVRH 355 Query: 360 GKAV 363 GK + Sbjct: 356 GKTL 359 Lambda K H 0.320 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 406 Number of extensions: 13 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 364 Length of database: 360 Length adjustment: 29 Effective length of query: 335 Effective length of database: 331 Effective search space: 110885 Effective search space used: 110885 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate GFF5324 PS417_27255 (glycine cleavage system protein T)
to HMM TIGR00528 (gcvT: glycine cleavage system T protein (EC 2.1.2.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00528.hmm # target sequence database: /tmp/gapView.17356.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00528 [M=362] Accession: TIGR00528 Description: gcvT: glycine cleavage system T protein Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.9e-129 415.4 0.0 1e-128 415.3 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF5324 PS417_27255 glycine cleavage sys Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF5324 PS417_27255 glycine cleavage system protein T # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 415.3 0.0 1e-128 1e-128 2 361 .. 3 354 .. 2 355 .. 0.95 Alignments for each domain: == domain 1 score: 415.3 bits; conditional E-value: 1e-128 TIGR00528 2 krtpLydlhtelggklvdfaGwelPvqykslieeheavrtkaGlfDvshmgkvelsGskslkfLqrllanDvdaL 76 +rtpLydlh lg+k+vdf+Gw +P++y+s++eeh+ vr + G+fDvshm+ ++++G+++ + Lq+llanDv++L lcl|FitnessBrowser__WCS417:GFF5324 3 QRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHQVRRDCGVFDVSHMTVIDVTGPQAKEWLQYLLANDVERL 77 79************************************************************************* PP TIGR00528 77 tk.GkaqysvllnaqGGvvDDlivykegednrfllvvnaataekDlewlkehleeevtldtlskeisllalqGPk 150 G+a+ys++ln +GGvvDD+ivy+++ ++lvvna+t+++D+ w++ +l + + + e+++la+qGP+ lcl|FitnessBrowser__WCS417:GFF5324 78 HDcGRALYSAMLNEKGGVVDDMIVYRTESA--YRLVVNASTRDQDMTWMHAQLGNYQVQLHERPELAMLAIQGPH 150 999**********************97766..***********************99999999***********9 PP TIGR00528 151 aktiledlldkaveg....lkefffvqeaelalkkaliartGytGedGfeiavanekavelwkklveaygvkPiG 221 a++ + +l+ ++ + lk+f + +++ +iartGytGedG ei+++ ++a +++ lv a g+ PiG lcl|FitnessBrowser__WCS417:GFF5324 151 ARQKIAELVTQSRSTlihqLKPFEGQADGDW-----FIARTGYTGEDGLEIVLPADQAPGFFNDLVGA-GISPIG 219 9988888888775554334899988877777.....********************************.****** PP TIGR00528 222 LgarDtLrleagmaLyGqeldeeitPleaglgwvvkkerkksdfiGravleeqkengtekklvGlemlekgiarn 296 LgarDtLrleagm+LyGq+++++i+Pl++++ w +++e+++++fiGra+le k+ g++ klvGl++ e+g++r lcl|FitnessBrowser__WCS417:GFF5324 220 LGARDTLRLEAGMNLYGQDIHQDISPLASNMAWSIAWEPAERNFIGRAALEAEKATGVQFKLVGLVLEERGVLRA 294 *************************************************************************** PP TIGR00528 297 elkvlltngekevGivtsGtlsPtLgknialayvdkelekiGtklevevrnklvkikvvkklfvr 361 + v+ ++ +G +tsG++sPtL k+iala+v++ +++eve+r+k ++++vvk+ fvr lcl|FitnessBrowser__WCS417:GFF5324 295 HQVVRI--ANIGEGEITSGSFSPTLSKSIALARVPTA---TADRAEVEIRGKWYPVRVVKPTFVR 354 *99999..5669***********************55...567899*****************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (362 nodes) Target sequences: 1 (360 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.39 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory