Align 2-aminomuconate deaminase; EC 3.5.99.5 (characterized)
to candidate GFF2376 PS417_12115 endoribonuclease L-PSP
Query= SwissProt::Q9KWS2 (142 letters) >FitnessBrowser__WCS417:GFF2376 Length = 129 Score = 50.1 bits (118), Expect = 1e-11 Identities = 30/78 (38%), Positives = 44/78 (56%), Gaps = 3/78 (3%) Query: 64 QTREVISNIRDILQSVGADLGDVVEVCSYLVNMNDFAAYNKVYAEFFDAT-GPARTTVAV 122 QTR+ + N+ IL+ G +L DVV V +L + DF ++NKV+ E+F PAR V Sbjct: 51 QTRQTMRNVVAILEEAGYELKDVVRVGVWLEDPRDFWSFNKVFGEYFTPEHAPARACVQA 110 Query: 123 HQLPHPQLVIEIKVVAYK 140 + + +EI VAYK Sbjct: 111 NMM--VDCKVEIDCVAYK 126 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 43 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 142 Length of database: 129 Length adjustment: 15 Effective length of query: 127 Effective length of database: 114 Effective search space: 14478 Effective search space used: 14478 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 42 (20.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory