GapMind for catabolism of small carbon sources

 

Alignments for a candidate for ech in Pseudomonas simiae WCS417

Align trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate GFF2712 PS417_13835 crotonase

Query= reanno::BFirm:BPHYT_RS17335
         (258 letters)



>FitnessBrowser__WCS417:GFF2712
          Length = 367

 Score = 94.0 bits (232), Expect = 4e-24
 Identities = 58/190 (30%), Positives = 96/190 (50%), Gaps = 6/190 (3%)

Query: 4   ENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGS-EKAFA 62
           + +L E R  +G +TLNRP  LNA+   ++  L + L+ +  D  + A+V+ G+ EKAF 
Sbjct: 17  DEVLAEVRNHIGHLTLNRPAGLNAITLNMVRRLASQLKAWADDPQVYAVVLRGAGEKAFC 76

Query: 63  AGADI-GMMSTYTYMDVYKGDYITRNWE---TVRSIRKPIIAAVAGFALGGGCELAMMCD 118
           AG DI  +  ++   D    D+    +     +   RKP++A + GF LGGG  L    D
Sbjct: 77  AGGDIRSLYDSFKNGDTLHQDFFVEEYALDLAIHHYRKPVLALMDGFVLGGGMGLVQGAD 136

Query: 119 IIFAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCLTARFMDAAEAERAGLVS 178
           +    + ++   PE+ +G  P  GG+  LPR +     + L +T   + AA+A   GL  
Sbjct: 137 LRVVTERSRLAMPEVAIGYFPDVGGSYFLPR-IPGELGIYLGVTGVQIRAADALYCGLAD 195

Query: 179 RVIPAASLVD 188
             + ++ L D
Sbjct: 196 WYLESSKLAD 205


Lambda     K      H
   0.321    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 258
Length of database: 367
Length adjustment: 27
Effective length of query: 231
Effective length of database: 340
Effective search space:    78540
Effective search space used:    78540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory