Align Xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate GFF2156 PS417_11000 dihydroxy-acid dehydratase
Query= reanno::pseudo6_N2E2:Pf6N2E2_1668 (594 letters) >FitnessBrowser__WCS417:GFF2156 Length = 578 Score = 444 bits (1142), Expect = e-129 Identities = 258/594 (43%), Positives = 354/594 (59%), Gaps = 25/594 (4%) Query: 6 KHRLRSEQWFNDPAHADMTALYVERYM-NYGMTREELQSGRPIIGIAQTGSDLTPCNRHH 64 K LRS QWF AD +M N G+ + G+PIIGI T S+LTPCN H Sbjct: 5 KPGLRSAQWFGT---ADKNGFMYRSWMKNQGIADHQFH-GKPIIGICNTWSELTPCNAHF 60 Query: 65 LELAQRVKAGIRDAGGIPMEFPVHPIAEQSRRPTAALDRNLAYLGLVEILHGYPLDGVVL 124 ++A+ VK G+ +AGG P+EFPV E + RPTA L RNLA + + E + G P+DGVVL Sbjct: 61 RQIAEHVKRGVIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEEAIRGNPIDGVVL 120 Query: 125 TTGCDKTTPACLMAAATTDLPAIVLSGGPMLDGHHKGELIGSGTVLWHARNLMAAGEIDY 184 TGCDKTTPA LM AA+ D+PAIV++GGPML+G HKG+ IGSGTV+W + AG I Sbjct: 121 LTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHKGQDIGSGTVVWQLSEQVKAGTITL 180 Query: 185 EGFMEMTTAASPSVGHCNTMGTALSMNALAEALGMSLPGCASIPAPYRERGQMAYATGKR 244 + F+ S S G CNTMGTA +M +AEALG SLP A+IPA R +A+ +G R Sbjct: 181 DDFLAAEGGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDARRYVLAHMSGMR 240 Query: 245 ICDLVRQDIRPSQIMTRQAFENAIAVASALGASSNCPPHLIAIARHMGVELSLEDWQRIG 304 ++VR+D++ S+I+T++AFENAI V +A+G S+N HL AIA +GVEL L+DW RIG Sbjct: 241 AVEMVREDLKLSKILTKEAFENAIRVNAAIGGSTNAVIHLKAIAGRIGVELDLDDWTRIG 300 Query: 305 EDVPLLVNCMPAGKYLGEGFHRAGGVPSVMHELQKAGRL-HEDCATVSGKTIGEIVSNS- 362 +P +V+ P+G++L E F+ AGG+P+V+ L +A + H + TV+GK++GE +S Sbjct: 301 RGMPTIVDLQPSGRFLMEEFYYAGGLPAVLRRLGEANLIPHPNALTVNGKSLGENTQDSP 360 Query: 363 LTSNTDVIHPFDTPLKHRAGFIVLSGNFFD-SAIMKMSVVGEAFRKTYLSEPGAENSFEA 421 + +VI D P++ G VL GN A++K S A + Sbjct: 361 IYGQDEVIRTLDNPIRADGGICVLRGNLAPLGAVLKPSAASPALMQ-----------HRG 409 Query: 422 RAIVFEGPEDYHARIDDPALDIDERCILVIRGVGTVGYPGSAEVVNMAPPAALIKQGIDS 481 RA+VFE + Y ARI+DP LD+D ILV++ G GYPG AEV NM PA L+ QG+ Sbjct: 410 RAVVFENFDMYKARINDPELDVDANSILVMKNCGPKGYPGMAEVGNMGLPAKLLAQGVTD 469 Query: 482 LPCLGDGRQSGTSASPSILNMSPEAAVGGGLALLKTNDRLKVDLNTRTVNLLIDDAEMAQ 541 + + D R SGT+ +L+++PEAA GG LA +K D +++D ++L I DAE+A Sbjct: 470 MVRISDARMSGTAYGTVVLHVAPEAAAGGPLATVKEGDWIELDCANGRLHLDIPDAELAA 529 Query: 542 RRREWIPNIPPSQTPWQELYRQLVGQLSTGGCLEPATLHLRVIARSGE-PRHSH 594 R + P +++LY V Q G V R E PRHSH Sbjct: 530 RMADLAPPQKLIVGGYRQLYIDHVLQADQG-----CDFDFLVGCRGAEVPRHSH 578 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 976 Number of extensions: 65 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 594 Length of database: 578 Length adjustment: 37 Effective length of query: 557 Effective length of database: 541 Effective search space: 301337 Effective search space used: 301337 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory