GapMind for catabolism of small carbon sources

 

Protein Ac3H11_2065 in Acidovorax sp. GW101-3H11

Annotation: FitnessBrowser__acidovorax_3H11:Ac3H11_2065

Length: 289 amino acids

Source: acidovorax_3H11 in FitnessBrowser

Candidate for 31 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
D-glucose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
lactose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
D-maltose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
sucrose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
trehalose catabolism gtsC hi Sugar ABC transporter permease (characterized, see rationale) 100% 100% 575.5 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
D-galactose catabolism PfGW456L13_1896 hi ABC transporter for D-Galactose and D-Glucose, permease component 2 (characterized) 61% 96% 338.2 ABC transporter for D-Cellobiose and D-Salicin, permease component 1 47% 280.8
D-xylose catabolism gtsC med ABC transporter for D-Glucose-6-Phosphate, permease component 1 (characterized) 61% 99% 347.1 ABC transporter for D-Galactose and D-Glucose, permease component 2 61% 338.2
D-cellobiose catabolism SMc04257 med ABC transporter for D-Cellobiose and D-Salicin, permease component 1 (characterized) 47% 94% 280.8 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-mannose catabolism TT_C0326 med Sugar transport system permease protein aka TT_C0326, component of The glucose/mannose porter TTC0326-8 plus MalK1 (ABC protein, shared with 3.A.1.1.25) (characterized) 48% 100% 266.2 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
L-arabinose catabolism araU lo AraU, component of Arabinose, fructose, xylose porter (characterized) 30% 98% 143.3 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-fructose catabolism araU lo AraU, component of Arabinose, fructose, xylose porter (characterized) 30% 98% 143.3 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
sucrose catabolism araU lo AraU, component of Arabinose, fructose, xylose porter (characterized) 30% 98% 143.3 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-xylose catabolism araU lo AraU, component of Arabinose, fructose, xylose porter (characterized) 30% 98% 143.3 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-cellobiose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-galactose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-glucose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
lactose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-maltose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-mannose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
sucrose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
trehalose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 30% 99% 136.7 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-maltose catabolism aglG lo ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 34% 57% 122.5 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
sucrose catabolism aglG lo ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 34% 57% 122.5 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
trehalose catabolism aglG lo ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 34% 57% 122.5 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-cellobiose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-glucose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
lactose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
D-maltose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
sucrose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1
trehalose catabolism aglG' lo Inner membrane ABC transporter permease protein (characterized, see rationale) 30% 56% 110.9 ABC transporter for D-Glucose-6-Phosphate, permease component 1 61% 347.1

Sequence Analysis Tools

View Ac3H11_2065 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MTAKPSLLPSVGRFLVYAVLALATAFFLLPLYAMLVTSFKYAEEIRSTSLLALPGSLNWS
AWGTAWQSACTGVDCNGLRPFFMNSVAMAVPAVLISTVWGALNGYVLSLWKFRGSDALFG
MLLFGVFMPFQVVLLPMSQVLGWLGLSSSITGLVLVHCLAGLAGTTLFFRNYYAAIPKEL
VNAARMDGASFFQIFWRIVLPLSTPIVMVTLIWQFTNIWNDFLFGVVFSGTDSKPVTVGL
NNLANTSSSVKAYNVDMAAAIIAGLPTMVIYVLAGKFFVRGLTAGAVKG

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory