Align Benzoate--CoA ligase; Benzoyl-CoA synthetase; EC 6.2.1.25 (characterized)
to candidate Ac3H11_3090 Benzoate-CoA ligase (EC 6.2.1.25)
Query= SwissProt::Q8GQN9 (527 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_3090 Length = 518 Score = 545 bits (1405), Expect = e-159 Identities = 289/515 (56%), Positives = 352/515 (68%), Gaps = 13/515 (2%) Query: 17 KIPERYNAADDLIGRNLLAGRGGKTVYIDDAGSYTYDELALRVNRCGSALRTTLGLQPKD 76 ++PE +N A+ L N RG + YIDD + +Y L R R + L G++ ++ Sbjct: 3 RLPEHFNFAEHLFALN--RARGDRMAYIDDHETLSYGALEDRARRLAAVLAAA-GVRREE 59 Query: 77 RVLVCVLDGIDFPTTFLGAIKGGVVPIAINTLLTESDYEYMLTDSAARVAVVSQELLPLF 136 RVL+ +LD D+P FLG + GV+P+A+NTLL DY YML+ + AR +VS LLP+ Sbjct: 60 RVLLLMLDTCDWPVAFLGCLYAGVLPVAVNTLLKPEDYAYMLSHARARAVLVSGALLPVL 119 Query: 137 APMLGKVPTLEHLVVAGG------AGEDSLAALLATGSEQFEAAPTRPDDHCFWLYSSGS 190 + + P L V AG + A +A A T PD FWLYSSGS Sbjct: 120 NDAMERQPHEVALQVVSQPQGPLPAGAVDMRAAIAAAVPLAAPAATGPDAPGFWLYSSGS 179 Query: 191 TGAPKGTVHIHSDLIHTAELYARPILGIREGDVVFSAAKLFFAYGLGNGLIFPLAVGATA 250 TG PKGTVH H +L TAELY +P+LG+ E D FSAAKL+FAYGLGN L FPL+VGAT Sbjct: 180 TGKPKGTVHTHGNLWWTAELYGKPVLGLTEQDRCFSAAKLYFAYGLGNSLTFPLSVGATV 239 Query: 251 VLMAERPTPAAVFERLRRHQPDIFYGVPTLYASMLANPDCPKEGELRLRACTSAGEALPE 310 VLMAERPTP A FER RHQP +F+G PT +A MLA+P P + LR C+SAGEALP Sbjct: 240 VLMAERPTPDATFERWTRHQPTVFFGAPTGFAGMLASPRLPPRSAVSLRMCSSAGEALPA 299 Query: 311 DVGRRWQARFGVDILDGIGSTEMLHIFLSNRAGDVHYGTSGKPVPGYRLRLIDEDGAEIT 370 ++ +R++A FG DI+DGIGSTEMLHIFLSNR GDV YGT+GKPVPGY + L EDG E+ Sbjct: 300 EIAQRFKAHFGCDIIDGIGSTEMLHIFLSNRPGDVRYGTTGKPVPGYEVSLRGEDGREVP 359 Query: 371 TAGVAGELQISGPSSAVMYWNNPEKTAATFMGEWTRSGDKYLVNDEGYYVYAGRSDDMLK 430 G G+L I GPS+A++YWNN EKT TF G WT+SGDKY+ + EGYY YAGRSDDMLK Sbjct: 360 D-GEIGDLYIQGPSAALLYWNNREKTRETFQGAWTKSGDKYVRDAEGYYTYAGRSDDMLK 418 Query: 431 VSGIYVSPIEVESALIAHEAVLEAAVVGWEDEDHLIKPKAFIVLKPGYGAGEALRTDLKA 490 VSGIYVSP EVE+ L+ H+AVLEAAV+G +D D L K KAF+VLK G A DLKA Sbjct: 419 VSGIYVSPFEVEATLMQHQAVLEAAVIGAQDADGLTKTKAFVVLKDGQ---TATPDDLKA 475 Query: 491 HVKNLLAPYKYPRWIEFVDDLPKTATGKIQRFKLR 525 VK LAPYKYPR IEFV +LPKTATGKIQRF+LR Sbjct: 476 FVKERLAPYKYPRLIEFVPELPKTATGKIQRFRLR 510 Lambda K H 0.319 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 823 Number of extensions: 32 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 527 Length of database: 518 Length adjustment: 35 Effective length of query: 492 Effective length of database: 483 Effective search space: 237636 Effective search space used: 237636 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory