Aligments for a candidate for praB in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for praB in Acidovorax sp. GW101-3H11

Align 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized)
to candidate Ac3H11_4184 Aldehyde dehydrogenase B (EC 1.2.1.22)

Query= metacyc::MONOMER-15108
         (486 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_4184
          Length = 498

 Score =  330 bits (847), Expect = 5e-95
 Identities = 182/473 (38%), Positives = 270/473 (57%), Gaps = 7/473 (1%)

Query: 15  IDGKFVPSLDGKTFDNINPATEEKLGTVAEGGAAEIDLAVQAAKKALNGPWKKMTANERI 74
           I+G++V  +    FD  +PAT  KL  VA  G A+ + A+ AA  A  GPWK  TA ER 
Sbjct: 23  INGQWV--VGSSRFDVNDPATGLKLADVANLGPADAEAAIAAANAAW-GPWKTKTAKERS 79

Query: 75  AVLRKVGDLILERKEELSVLESLDTGKPTWLSGSIDIPRAAYNFHFFSDYIRTITNEAT- 133
            +LRK  DL++  +++L  + + + GKP       ++   A    +F++  + I  E   
Sbjct: 80  IILRKWFDLLMANQDDLGRIMTAEQGKPL-AEAKGEVAYGASFVEWFAEEAKRINGETLP 138

Query: 134 QMDDVALNYAIRRPVGVIGLINPWNLPLLLMTWKLAPALAAGNTVVMKPAELTPMTATVL 193
           Q D+      +++P+GV   I PWN PL ++T K+APALAAG  VV+KPAELTP+TA   
Sbjct: 139 QFDNNRRLMVLKQPIGVCAAITPWNFPLAMITRKVAPALAAGCPVVIKPAELTPLTALAA 198

Query: 194 AEICRDAGVPDGVVNLVHGFGPNSA--GAALTEHPDVNAISFTGETTTGKIIMASAAKTL 251
           AE+   AG+P GV N++     NS   G  L     V  ISFTG T  G+I+MA +A T+
Sbjct: 199 AELAIRAGIPAGVFNILPADSDNSIAIGKVLCASDVVRHISFTGSTEVGRILMAQSAPTV 258

Query: 252 KRLSYELGGKNPNVIFADSNLDEVIETTMKSSFINQGEVCLCGSRIYVERPAYEAFLEKF 311
           K++S ELGG  P ++F D+++D  +E    S + N G+ C+C +R YV+   Y+ F+ KF
Sbjct: 259 KKMSLELGGNAPFIVFDDADIDSAVEGAFASKYRNAGQTCVCTNRFYVQEGVYDEFVAKF 318

Query: 312 VAKTKELVVGDPFDAKTKVGALISDEHYERVTGYIKLAVEEGGTILTGGKRPEGLEKGYF 371
            AK K   VG+ F+A    G LI +    +V  ++  A+ +GG ++ GG+R   L  G F
Sbjct: 319 AAKVKTAKVGNGFEAGVNQGPLIEEAALTKVQRHVDDALAKGGQVVAGGQRLTALGSGQF 378

Query: 372 LEPTIITGLTRDCRVVKEEIFGPVVTVIPFDTEEEVLEQINDTHYGLSASVWTNDLRRAH 431
            EPT++   T D    +EE FGP   V  F TE+E ++  N+T +GL++  ++ D+ R  
Sbjct: 379 FEPTVVANATADMLCAREETFGPFAPVFKFKTEQEAIDAANNTEFGLASYFYSRDVGRIF 438

Query: 432 RVAGQIEAGIVWVNTWFLRDLRTPFGGMKQSGIGREGGLHSFEFYSELTNICI 484
           RV   +E G+V  N   L     PFGG+KQSG+GREG  H  + Y E+  +C+
Sbjct: 439 RVTEALEYGMVGANVGILATEHVPFGGVKQSGLGREGSHHGMDDYVEIKYLCL 491


Lambda     K      H
   0.318    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 608
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 486
Length of database: 498
Length adjustment: 34
Effective length of query: 452
Effective length of database: 464
Effective search space:   209728
Effective search space used:   209728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources