Align ABC transporter for D-Alanine, permease component 1 (characterized)
to candidate Ac3H11_3326 Amino acid ABC transporter, permease protein
Query= reanno::pseudo6_N2E2:Pf6N2E2_5404 (365 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_3326 Length = 260 Score = 102 bits (255), Expect = 9e-27 Identities = 63/168 (37%), Positives = 91/168 (54%), Gaps = 9/168 (5%) Query: 148 DAVATSQWGGLMLTLVIATVGIVGALPLGIVLALGRRSNMPAIRVVCVTFIEFWRGVPLI 207 D T+ W LTL+ +VG+V LG AL R + +R +I RG PL+ Sbjct: 54 DGARTTLW----LTLISGSVGLV----LGTGAALARTARWAVVRWAASFYIWVIRGTPLL 105 Query: 208 TVLFMSSVMLPLFLPEGMNFDKLLRALIGVILFQSAYIAEVVRGGLQAIPKGQYEAAAAM 267 + LP+ +P G+N A++ + L AY AE +R GL A+P+GQ EAA A+ Sbjct: 106 VQILFVYFALPVLVP-GLNLPDFAAAVLALGLNVGAYNAEAIRAGLLAVPRGQTEAAKAL 164 Query: 268 GLGYWRSMGLVILPQALKLVIPGIVNTFIALFKDTSLVIIIGLFDLLN 315 GLG V+ PQA K+ +P +V+ F+AL KD+SL IG+ +L N Sbjct: 165 GLGRMHVFFDVVFPQAFKISLPPLVSNFVALLKDSSLAYAIGVVELTN 212 Lambda K H 0.330 0.144 0.469 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 301 Number of extensions: 20 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 365 Length of database: 260 Length adjustment: 27 Effective length of query: 338 Effective length of database: 233 Effective search space: 78754 Effective search space used: 78754 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory