Alignments for a candidate for araVsh in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for araVsh in Acidovorax sp. GW101-3H11

Align ABC transporter related (characterized, see rationale)
to candidate Ac3H11_607 Predicted L-arabinose ABC transport system, ATP-binding protein

Query= uniprot:A0KWY5
         (499 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_607
          Length = 517

 Score =  453 bits (1165), Expect = e-132
 Identities = 242/484 (50%), Positives = 334/484 (69%), Gaps = 7/484 (1%)

Query: 4   ILELKQISKHYPGVKALEDVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQSKDMGDILF 63
           +L+L  I K + G+  L DV L L+ GE+HAL+G+NGAGKSTL+KV+TG      G +  
Sbjct: 18  VLQLSGIHKQFAGITVLRDVQLNLYPGEIHALMGQNGAGKSTLIKVLTGVLEASGGQMRL 77

Query: 64  LGEPQHFNTPMDAQKAGISTVYQEVNLVPNLTVAQNLFLGYEPRRLGL-----IHFKKMY 118
            G+    ++P+ AQ+ GISTVYQEVNL PNL+VA+N+F G  PR  G+     I +  ++
Sbjct: 78  GGQAVWPDSPLAAQRLGISTVYQEVNLCPNLSVAENIFAGRYPR-CGIAQGFRIDWATLH 136

Query: 119 ADARAVLTQFKLDIDVSAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASLDAKEVQV 178
             AR ++ +  L IDV+  LSDY +AVQQL+AIAR +++ ++VL+LDEPT+SLD  EVQ 
Sbjct: 137 QRARDLVARIGLQIDVTRLLSDYPVAVQQLVAIARALSIESRVLILDEPTSSLDDDEVQK 196

Query: 179 LFGILNQLKAKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPKLIEAMLG 238
           LF +L +L+++G++IVF+THFL+QVY +SDRITVLRNG ++GE+L  +L    LI AMLG
Sbjct: 197 LFEVLRRLRSEGLSIVFVTHFLNQVYAVSDRITVLRNGSWVGEWLAKDLGPQALIAAMLG 256

Query: 239 RSLQEQLVDKQEKERTVTRAEAVLLSLEDVSVKGSIQSMNLTVPKGQAVGLAGLLGSGRS 298
           R L     ++      V    A LL  E +     +Q ++L +  G+ VGLAGLLGSGR+
Sbjct: 257 RDLAAAS-EQPAPAPAVDSRHANLLQAEGLGQDTQLQPLDLQIRAGEVVGLAGLLGSGRT 315

Query: 299 EVCNAVFGLDLVDSGSIHLAGQKLNLSQPVDAISAGIALCPEDRKIDGIIGPLSIRENII 358
           E+   +FGL+  D G++ + GQ +  + P+DAI  G+ALCPE+RK DGI+  LS+RENI 
Sbjct: 316 ELARLLFGLEQPDRGALRIDGQVVKFANPMDAIRHGLALCPEERKTDGIVAELSVRENIA 375

Query: 359 LALQARIGWWRYLSNTRQQEIAQFFIDKLQIATPDADKPIEQLSGGNQQKVILARWLAIE 418
           LALQAR+G  ++LS + Q E+A+ ++  L I T   DKPI  LSGGNQQK ILARW+AIE
Sbjct: 376 LALQARMGVGKFLSRSEQTELAERYVKLLGIKTETVDKPIGLLSGGNQQKAILARWMAIE 435

Query: 419 PILLVLDEPTRGIDIGAHAEIVKLIRTLCDEGMSLLVASSELDELVAFSNKVVVLRDRYA 478
           P LL+LDEPTRGID+ A  EI+  I  L   GM++L  SSE+ E+V  ++++VVLRDR  
Sbjct: 436 PRLLILDEPTRGIDVAAKQEIMDQILRLAQAGMAVLFISSEMSEVVRVAHRIVVLRDRRK 495

Query: 479 VREL 482
           V EL
Sbjct: 496 VGEL 499



 Score = 72.8 bits (177), Expect = 3e-17
 Identities = 62/237 (26%), Positives = 111/237 (46%), Gaps = 30/237 (12%)

Query: 274 IQSMNLTVPKGQAVGLAGLLGSGRSEVCNAVFGLDLVDSGSIHLAGQKLNLSQPVDAISA 333
           ++ + L +  G+   L G  G+G+S +   + G+     G + L GQ +    P+ A   
Sbjct: 34  LRDVQLNLYPGEIHALMGQNGAGKSTLIKVLTGVLEASGGQMRLGGQAVWPDSPLAAQRL 93

Query: 334 GIA-------LCPEDRKIDGIIGPLSIRENIILALQARIGW-------WRYLSNTRQQEI 379
           GI+       LCP           LS+ ENI      R G        W  L    +  +
Sbjct: 94  GISTVYQEVNLCPN----------LSVAENIFAGRYPRCGIAQGFRIDWATLHQRARDLV 143

Query: 380 AQFFIDKLQIATPDADKPIEQLSGGNQQKVILARWLAIEPILLVLDEPTRGIDIGAHAEI 439
           A+  + ++ +    +D P+       QQ V +AR L+IE  +L+LDEPT  +D     ++
Sbjct: 144 ARIGL-QIDVTRLLSDYPVAV-----QQLVAIARALSIESRVLILDEPTSSLDDDEVQKL 197

Query: 440 VKLIRTLCDEGMSLLVASSELDELVAFSNKVVVLRDRYAVRELSGAELTSQHVMQAI 496
            +++R L  EG+S++  +  L+++ A S+++ VLR+   V E    +L  Q ++ A+
Sbjct: 198 FEVLRRLRSEGLSIVFVTHFLNQVYAVSDRITVLRNGSWVGEWLAKDLGPQALIAAM 254


Lambda     K      H
   0.319    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 701
Number of extensions: 26
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 499
Length of database: 517
Length adjustment: 34
Effective length of query: 465
Effective length of database: 483
Effective search space:   224595
Effective search space used:   224595
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources