Alignments for a candidate for araVsh in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for araVsh in Acidovorax sp. GW101-3H11

Align ABC transporter related (characterized, see rationale)
to candidate Ac3H11_609 L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)

Query= uniprot:A0KWY5
         (499 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_609
          Length = 505

 Score =  325 bits (832), Expect = 3e-93
 Identities = 183/503 (36%), Positives = 308/503 (61%), Gaps = 7/503 (1%)

Query: 3   LILELKQISKHYPGVKALEDVSLRLFAGEVHALLGENGAGKSTLVKVMTGA--QSKDMGD 60
           ++LE++ I K +PGV AL  V+L++ AGE+HA++GENGAGKSTL+KV++G        G 
Sbjct: 1   MLLEMRNIRKTFPGVVALNQVNLQVQAGEIHAIVGENGAGKSTLMKVLSGVYPHGSYSGQ 60

Query: 61  ILFLGEPQHFNTPMDAQKAGISTVYQEVNLVPNLTVAQNLFLGYEPRRLGLIHFKKMYAD 120
           ILF G+ + F    D++  GI  ++QE+ LVP L++A+N+FLG E  R G+I +   ++ 
Sbjct: 61  ILFDGQEREFAGIRDSEHLGIIIIHQELALVPLLSIAENIFLGNETARHGVIDWMAAHSR 120

Query: 121 ARAVLTQFKLDIDVSAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASLDAKEVQVLF 180
           A+A+L +  L      P+    +  QQL+ IA+ ++   ++L+LDEPTASL+  + Q L 
Sbjct: 121 AQALLHKVGLGESPDTPVGQLGVGKQQLVEIAKALSRKVRLLILDEPTASLNENDSQALL 180

Query: 181 GILNQLKAKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELP--QPKLIEAMLG 238
            +L +LKA+G+  + I+H L+++ +++D ITVLR+G  +      E P  + ++I+AM+G
Sbjct: 181 DLLLELKAQGITCILISHKLNEISRVADAITVLRDGSTVQMLDCREGPVSEDRVIQAMVG 240

Query: 239 RSLQEQLVDKQEKE-RTVTRAEAVLLSLEDVSVKGSIQSMNLTVPKGQAVGLAGLLGSGR 297
           R + ++   +Q +    V             S +  ++ ++L V +G+ VG+AGL+G+GR
Sbjct: 241 REMSDRYPQRQPQVGEIVFEVRNWRAHHPQRSDREHLKGIDLNVRRGEIVGIAGLMGAGR 300

Query: 298 SEVCNAVFGLDLVD--SGSIHLAGQKLNLSQPVDAISAGIALCPEDRKIDGIIGPLSIRE 355
           +E+  ++FG       SG + L GQ +++S    A+S G+A   EDRK +G++    I+ 
Sbjct: 301 TELAMSIFGRSWGQRISGEVRLHGQPIDVSTVEKAVSHGLAYVTEDRKGNGLVLNEDIQF 360

Query: 356 NIILALQARIGWWRYLSNTRQQEIAQFFIDKLQIATPDADKPIEQLSGGNQQKVILARWL 415
           N  LA    + +   + + ++  +AQ + +KL+I     D+    LSGGNQQKV+L++WL
Sbjct: 361 NTSLANLPGVSFASVIDSGQEHRVAQDYREKLRIRCSGVDQKTLNLSGGNQQKVVLSKWL 420

Query: 416 AIEPILLVLDEPTRGIDIGAHAEIVKLIRTLCDEGMSLLVASSELDELVAFSNKVVVLRD 475
              P +L+LDEPTRGID+GA  EI  LI  L  EG  ++V SSE+ EL+  ++++ V+ +
Sbjct: 421 FTSPEVLILDEPTRGIDVGAKYEIYTLIAQLAAEGKCVIVISSEMPELLGITDRIYVMNE 480

Query: 476 RYAVRELSGAELTSQHVMQAIAE 498
              V E+  +E + + +M+AI +
Sbjct: 481 GRFVAEMPTSEASQEKIMRAIVK 503



 Score = 73.2 bits (178), Expect = 2e-17
 Identities = 60/249 (24%), Positives = 120/249 (48%), Gaps = 11/249 (4%)

Query: 3   LILELKQISKHYP---GVKALEDVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQ--SKD 57
           ++ E++    H+P     + L+ + L +  GE+  + G  GAG++ L   + G     + 
Sbjct: 257 IVFEVRNWRAHHPQRSDREHLKGIDLNVRRGEIVGIAGLMGAGRTELAMSIFGRSWGQRI 316

Query: 58  MGDILFLGEPQHFNTPMDAQKAGISTVYQEVN---LVPNLTVAQNLFLGYEP--RRLGLI 112
            G++   G+P   +T   A   G++ V ++     LV N  +  N  L   P      +I
Sbjct: 317 SGEVRLHGQPIDVSTVEKAVSHGLAYVTEDRKGNGLVLNEDIQFNTSLANLPGVSFASVI 376

Query: 113 HFKKMYADARAVLTQFKLDID-VSAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASL 171
              + +  A+    + ++    V     + S   QQ + +++ +  S +VL+LDEPT  +
Sbjct: 377 DSGQEHRVAQDYREKLRIRCSGVDQKTLNLSGGNQQKVVLSKWLFTSPEVLILDEPTRGI 436

Query: 172 DAKEVQVLFGILNQLKAKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPK 231
           D      ++ ++ QL A+G  ++ I+  + ++  I+DRI V+  G+F+ E  T+E  Q K
Sbjct: 437 DVGAKYEIYTLIAQLAAEGKCVIVISSEMPELLGITDRIYVMNEGRFVAEMPTSEASQEK 496

Query: 232 LIEAMLGRS 240
           ++ A++  S
Sbjct: 497 IMRAIVKAS 505


Lambda     K      H
   0.319    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 586
Number of extensions: 35
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 499
Length of database: 505
Length adjustment: 34
Effective length of query: 465
Effective length of database: 471
Effective search space:   219015
Effective search space used:   219015
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources