GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bztD in Acidovorax sp. GW101-3H11

Align BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized)
to candidate Ac3H11_1958 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4)

Query= TCDB::Q52666
         (263 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_1958
          Length = 245

 Score =  271 bits (694), Expect = 7e-78
 Identities = 137/244 (56%), Positives = 180/244 (73%), Gaps = 3/244 (1%)

Query: 23  IQISQMNKWYGQFHVLRDINLTVHRGERIVIAGPSGSGKSTMIRCINRLEEHQSGKIIVD 82
           I++  ++KWYG   VL + + T+++GE +V+ GPSGSGKST+I+ IN LE  Q G+I VD
Sbjct: 2   IELKNVSKWYGPVQVLNECSATINKGEVVVVCGPSGSGKSTLIKTINALEPFQKGEITVD 61

Query: 83  GIELTSDLKNIDKVRSEVGMVFQHFNLFPHLTILENLTLAPIWVRKVPKREAEETAMYYL 142
           G++L     N+ K+RS VGMVFQHF LFPHL++ +NLT+A I V      EA++  +  L
Sbjct: 62  GVKLHDPSTNLPKLRSRVGMVFQHFELFPHLSVTDNLTIAQIKVLGRSADEAKKRGLKML 121

Query: 143 EKVKIPEQAQKYPGQLSGGQQQRVAIARSLCMKPKIMLFDEPTSALDPEMIKEVLDTMIQ 202
           E+V +     K+PGQLSGGQQQRVAIAR+L M P +MLFDEPTSALDPEM+ EVLD M+ 
Sbjct: 122 ERVGLIAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLFDEPTSALDPEMVGEVLDVMVG 181

Query: 203 LAEEGMTMLCVTHEMGFAQAVANRVIFM-ADGQIVEQNNPHDFFHNPQSE--RTKQFLSQ 259
           LA EGMTM+CVTHEMGFA+ V+NRVIFM   G+I+E  +  +FF+NP +   RTK FL++
Sbjct: 182 LANEGMTMMCVTHEMGFARKVSNRVIFMDVGGKILEDCSKDEFFNNPDARQPRTKDFLNK 241

Query: 260 ILGH 263
           IL H
Sbjct: 242 ILAH 245


Lambda     K      H
   0.320    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 208
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 263
Length of database: 245
Length adjustment: 24
Effective length of query: 239
Effective length of database: 221
Effective search space:    52819
Effective search space used:    52819
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory