GapMind for catabolism of small carbon sources

 

Aligments for a candidate for PS417_17605 in Acidovorax sp. GW101-3H11

Align ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale)
to candidate Ac3H11_3327 Glutamine transport ATP-binding protein GlnQ (TC 3.A.1.3.2)

Query= uniprot:A0A1N7U8S3
         (276 letters)



>lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3327 Glutamine transport
           ATP-binding protein GlnQ (TC 3.A.1.3.2)
          Length = 249

 Score =  222 bits (566), Expect = 5e-63
 Identities = 121/244 (49%), Positives = 164/244 (67%), Gaps = 12/244 (4%)

Query: 29  VEGIHKRYGEHEVLKGVSLNARQGDVISLIGASGSGKSTMLRCINFLEQPDAGVITLDGI 88
           V+ + K +G H+VLK VS     G+V  +IGASGSGKST+LR IN LE  D+G IT+ G 
Sbjct: 12  VDRVCKSFGAHQVLKDVSTTFNTGEVTVIIGASGSGKSTLLRAINRLEPHDSGTITIGGE 71

Query: 89  SIEMRQGRAGTRAPHQDQLQNLRTRLAMVFQHFNLWSHMTVLENITMAPRRVLDVSAAEA 148
            +             Q  LQ  R+ + MVFQ FNL+ HM+VL+N+T+APRR+      +A
Sbjct: 72  PV----------GDDQHLLQKQRSEVGMVFQQFNLFGHMSVLDNVTLAPRRIRHTPRTQA 121

Query: 149 EKRARMYLDKVGLPSRVADQYPAFLSGGQQQRVAIARALAMEPEIILFDEPTSALDPELV 208
             +A   L +VG+    A +YP  LSGGQQQRVAIARALAM+P+++LFDEPTSALDPE+V
Sbjct: 122 NAQALALLTRVGMQDH-AHKYPWQLSGGQQQRVAIARALAMQPKVMLFDEPTSALDPEMV 180

Query: 209 GEVLKVIQTLAEEGRTMLMVTHEMGFARQVSSQVLFLHQGRV-EEHGDARILDQPNSERL 267
            EVL V++ LA  G TM++VTHEMGFAR+VS +V+F  QGR+  +   A   + P ++R+
Sbjct: 181 QEVLDVMRELARGGMTMIVVTHEMGFAREVSDRVMFFDQGRIAHDAPPAEFFNNPANDRI 240

Query: 268 QQFL 271
           + F+
Sbjct: 241 RAFI 244


Lambda     K      H
   0.319    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 218
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 276
Length of database: 249
Length adjustment: 25
Effective length of query: 251
Effective length of database: 224
Effective search space:    56224
Effective search space used:    56224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory