Aligments for a candidate for mglA in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for mglA in Acidovorax sp. GW101-3H11

Align Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized)
to candidate Ac3H11_2881 Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1)

Query= TCDB::P0AAG8
         (506 letters)



>lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2881 Ribose ABC
           transport system, ATP-binding protein RbsA (TC
           3.A.1.2.1)
          Length = 496

 Score =  322 bits (826), Expect = 1e-92
 Identities = 178/493 (36%), Positives = 284/493 (57%), Gaps = 7/493 (1%)

Query: 14  LEMSGINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTILFQ 73
           +E   + K F  V+ L  V   ++P  ++ L+GENGAGKSTL+K L G     +G ++  
Sbjct: 5   VEFRNVTKEFGPVRVLHGVGFALQPGRVYGLLGENGAGKSTLMKILAGYESPTTGEVVVD 64

Query: 74  GKEIDFHSAKEALE-NGISMVHQELNLVLQRSVMDNMWLGRYPTKGMFVDQDKMYRETKA 132
           G          A E  GI ++HQE NL    ++  N++LG    +G+F+D   M  +T+ 
Sbjct: 65  GAVRAPGGGSRAAEAQGIVLIHQEFNLADDLTIAQNIFLGHEIKRGLFLDDKAMREKTRE 124

Query: 133 IFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHLFTII 192
              ++ + +DP  RV  L V++ Q++EIA+A + NA+++IMDEPT++LT  E   LF ++
Sbjct: 125 ALAKVGLPLDPDTRVRKLIVAEKQLVEIARALARNARLLIMDEPTATLTPGETERLFALM 184

Query: 193 RKLKERGCGIVYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQ 252
             LK  G  I+YISHK++E+ +  DEV V+RDG  +A E  A +T  ++  +MVGR L  
Sbjct: 185 AGLKAAGVTIIYISHKLDEVERTTDEVVVMRDGLLVAREATASVTRRQMANLMVGRELAD 244

Query: 253 RFPDKENKP--GEVILEVRNLTSLRQPSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLF 310
            FP K   P  G   + VR LT         V F++ +GEILG AGLVGA RT++ E L 
Sbjct: 245 LFPPKLPAPQDGAPAITVRGLTV--PGWAEGVDFEVRRGEILGFAGLVGAGRTELFEGLL 302

Query: 311 GIREKSAGTITLHGKQINNHNANEAINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNY 370
           G+R ++AGT+ + G+ +   +  +A  HG   ++E+R+  G++ +  +  N  +  +  Y
Sbjct: 303 GLRPRTAGTVEIAGQPVQLKSPRDAARHGLTYLSEDRKGKGLHVHFGLRPNLTLMALERY 362

Query: 371 KNKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILML 430
                 LD +  ++  +  +    ++T     +  SLSGGNQQK+ + + L   P +++L
Sbjct: 363 AKP--WLDPAAEQAALREAVQEFGIRTGSLEVRASSLSGGNQQKLALAKVLHPGPSVVVL 420

Query: 431 DEPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDT 490
           DEPTRG+DVGAK EIY L+  LA++G  +I+ISSE+ EL+G+  R+ VM  G +   +  
Sbjct: 421 DEPTRGVDVGAKREIYHLVQRLAEQGLAVIVISSELMELIGLCHRVAVMRAGRLQTTLQE 480

Query: 491 KTTTQNEILRLAS 503
              T+ E++  A+
Sbjct: 481 PHLTEEELIAHAT 493



 Score = 75.5 bits (184), Expect = 4e-18
 Identities = 55/225 (24%), Positives = 104/225 (46%), Gaps = 9/225 (4%)

Query: 30  DNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENG 89
           + V+ +VR   I    G  GAG++ L + L G+  + +GT+   G+ +   S ++A  +G
Sbjct: 272 EGVDFEVRRGEILGFAGLVGAGRTELFEGLLGLRPRTAGTVEIAGQPVQLKSPRDAARHG 331

Query: 90  ISMVHQELN---LVLQRSVMDN---MWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDP 143
           ++ + ++     L +   +  N   M L RY     ++D        +    E  I    
Sbjct: 332 LTYLSEDRKGKGLHVHFGLRPNLTLMALERYAKP--WLDPAAEQAALREAVQEFGIRTGS 389

Query: 144 -RARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGI 202
              R  +LS    Q + +AK       +V++DEPT  +       ++ ++++L E+G  +
Sbjct: 390 LEVRASSLSGGNQQKLALAKVLHPGPSVVVLDEPTRGVDVGAKREIYHLVQRLAEQGLAV 449

Query: 203 VYISHKMEEIFQLCDEVTVLRDGQWIATEPLAGLTMDKIIAMMVG 247
           + IS ++ E+  LC  V V+R G+   T     LT +++IA   G
Sbjct: 450 IVISSELMELIGLCHRVAVMRAGRLQTTLQEPHLTEEELIAHATG 494


Lambda     K      H
   0.318    0.136    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 534
Number of extensions: 19
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 496
Length adjustment: 34
Effective length of query: 472
Effective length of database: 462
Effective search space:   218064
Effective search space used:   218064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources