Aligments for a candidate for gdhA in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for gdhA in Acidovorax sp. GW101-3H11

Align NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2; Surface-associated protein PGAG1 (uncharacterized)
to candidate Ac3H11_315 NADP-specific glutamate dehydrogenase (EC 1.4.1.4)

Query= curated2:B2RKJ1
         (445 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_315
          Length = 464

 Score =  502 bits (1292), Expect = e-146
 Identities = 252/434 (58%), Positives = 317/434 (73%), Gaps = 5/434 (1%)

Query: 13  KHPGESEFLQAVKEVLLSVEEVYNQHPEFEKNGIIERIVEPDRVFTFRVPWVDDQGKVQV 72
           ++PG+ EFLQAV EV+ S+     +HP + ++G++ER+VEP+R+  FRV WVDD G VQV
Sbjct: 33  RNPGQPEFLQAVTEVMESLWPFIAEHPRYAEHGLLERLVEPERIVMFRVSWVDDHGTVQV 92

Query: 73  NIGYRVQFNNAIGPYKGGIRFHPSVNLSILKFLGFEQMFKNALTTLPMGGGKGGADFSPK 132
           N GYR+Q + AIGP+KGGIRFHPSVNLS+LKFL FEQ FKNALTTLPMGGGKGG+DF PK
Sbjct: 93  NRGYRIQHSMAIGPFKGGIRFHPSVNLSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPK 152

Query: 133 GKSEAEIMRFCQSFMTELWRNIGPDTDIPAGDIGVGGREVGYMFGMYKKLAREHTGTLTG 192
           G+S  E+MRFCQ+F++EL+R++G DTD+PAGDIGVGGREVG+M GM KKL+       TG
Sbjct: 153 GRSPGEVMRFCQAFVSELFRHVGADTDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTG 212

Query: 193 KGFEFGGSRLRPESTGFGAVYFVQNMCKQNGVDYKGKTLAISGFGNVAWGVAQKATELGI 252
           KG  FGGS +RPE+TG+G VYF Q M + NG    G  +++SG GNVA    +KA +LG 
Sbjct: 213 KGLSFGGSLIRPEATGYGTVYFAQEMLRANGRSLDGLRVSVSGSGNVAQYAVEKALQLGA 272

Query: 253 KVVTISGPDGYVYDPDGINTPEKFRCMLDLRDSGNDVVSDYVKRFPNAQFFPGKKPWEQK 312
           KV+T+S   G + D DG  TPEK   ++D+++     VSDY +R    +F  G +PW   
Sbjct: 273 KVITVSDSSGTIVDEDGF-TPEKLAILMDVKNHHYGRVSDYAER-TGVKFEAGVRPWHVP 330

Query: 313 VDFAMPCATQNEMNLEDAKTLHKNGVTLVAETSNMGCTAEASEYYVANKMLFAPGKAVNA 372
           VD A+PCATQNE++  DA TL KNGV  VAE +NM  T EA++ + A  +L+APGKA NA
Sbjct: 331 VDVALPCATQNELDARDAATLIKNGVVCVAEGANMPSTIEAAKVFEAAGVLYAPGKASNA 390

Query: 373 GGVSCSGLEMTQNAMHLVWTNEEVDKWLHQIMQDIHEQCVTYGK--DGNYIDYVKGANIA 430
           GGV+ SGLEM+QNA  L W  EEVD  L QIMQ IH  C+ YGK  DG  + YV GANIA
Sbjct: 391 GGVATSGLEMSQNAARLAWPREEVDARLLQIMQGIHAACLRYGKHADGR-VSYVDGANIA 449

Query: 431 GFMKVAKAMVAQGV 444
           GF+KVA AM+AQGV
Sbjct: 450 GFVKVADAMLAQGV 463


Lambda     K      H
   0.319    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 617
Number of extensions: 25
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 445
Length of database: 464
Length adjustment: 33
Effective length of query: 412
Effective length of database: 431
Effective search space:   177572
Effective search space used:   177572
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources