Alignments for a candidate for tpi in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for tpi in Acidovorax sp. GW101-3H11

Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate Ac3H11_1454 Triosephosphate isomerase (EC 5.3.1.1)

Query= BRENDA::Q10657
         (247 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_1454
          Length = 249

 Score =  223 bits (568), Expect = 3e-63
 Identities = 128/247 (51%), Positives = 166/247 (67%), Gaps = 7/247 (2%)

Query: 3   RKFFVGGNWKMNGDYASVDGIVTFLNASADNSSVDVVVAPPAPYLAYAKSKLKAG--VLV 60
           +K  + GNWKMNG  A+ + ++  L A   + + DV VA PAPYLA  ++ L AG  V V
Sbjct: 4   KKKLIAGNWKMNGSLAANEVLLKALIAGLGDVTCDVAVAVPAPYLAQVQA-LTAGTAVAV 62

Query: 61  AAQNCYKVPKGAFTGEISPAMIKDLGLEWVILGHSERRHVFGESDALIAEKTVHALEAGI 120
           AAQ+  +   GA+TGE+S AM+KD G+ + ++GHSERR   GE+D  +AEK   AL AG+
Sbjct: 63  AAQDVSRYESGAYTGEVSVAMLKDFGVRYALVGHSERRQYHGETDVAVAEKAQRALAAGV 122

Query: 121 KVVFCIGEKLEEREAGHTKDVNFRQLQAIVD-KGVSWENIVIAYEPVWAIGTGKTASGEQ 179
             + C+GE LEEREAG T+ V  RQL A++   G     IV+AYEPVWAIGTG+TAS EQ
Sbjct: 123 TPIVCVGETLEEREAGQTEAVVKRQLAAVIHLNGHCISEIVVAYEPVWAIGTGRTASPEQ 182

Query: 180 AQEVHEWIRAFLKEKVSPAVADATRIIYGGSVTADNAAELGKKPDIDGFLVGGASLK-PD 238
           AQ VH  +RA L    +   AD  R++YGGS+ A NAA+L  +PDIDG LVGGASLK  D
Sbjct: 183 AQAVHAVLRAQL--AAASEHADRIRLLYGGSMNAGNAAQLLAQPDIDGGLVGGASLKAAD 240

Query: 239 FVKIINA 245
           F++II A
Sbjct: 241 FLQIIAA 247


Lambda     K      H
   0.317    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 187
Number of extensions: 11
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 247
Length of database: 249
Length adjustment: 24
Effective length of query: 223
Effective length of database: 225
Effective search space:    50175
Effective search space used:    50175
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 46 (22.3 bits)

Align candidate Ac3H11_1454 (Triosephosphate isomerase (EC 5.3.1.1))
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00419.hmm
# target sequence database:        /tmp/gapView.20142.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00419  [M=228]
Accession:   TIGR00419
Description: tim: triose-phosphate isomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                        -----------
    6.7e-67  211.6   6.4    7.8e-67  211.4   6.4    1.0  1  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454  Triosephosphate isomerase (EC 5.


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454  Triosephosphate isomerase (EC 5.3.1.1)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  211.4   6.4   7.8e-67   7.8e-67       1     227 [.       7     238 ..       7     239 .. 0.94

  Alignments for each domain:
  == domain 1  score: 211.4 bits;  conditional E-value: 7.8e-67
                                        TIGR00419   1 lviinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeve.seiqvaAqnv 61 
                                                      l+ +n+K+n+s+   e ++  l + + ++  + vava+p  +l  v+     + + vaAq+v
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454   7 LIAGNWKMNGSLAANEVLLKALIAGLGDV-TCDVAVAVPAPYLAQVQALTAgTAVAVAAQDV 67 
                                                      689**********************9987.599999999999999998777799******** PP

                                        TIGR00419  62 davksGaftGeisAemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvg 123
                                                         +sGa+tGe+s +mlkd+G+++ l+gHsErR ++ e+d  +++k  r+ + g++++vCvg
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454  68 SRYESGAYTGEVSVAMLKDFGVRYALVGHSERRQYHGETDVAVAEKAQRALAAGVTPIVCVG 129
                                                      ************************************************************** PP

                                        TIGR00419 124 etleereaartinnvattaaaaA......lepdvvAvEPveliGtGkpvskAeaevveksvr 179
                                                      etleerea++t  +v+++ aa+       +++ vvA+EPv++iGtG+++s+ +a++v++ +r
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454 130 ETLEEREAGQTEAVVKRQLAAVIhlnghcISEIVVAYEPVWAIGTGRTASPEQAQAVHAVLR 191
                                                      *********998888888777768999989999***************************** PP

                                        TIGR00419 180 dhlkkvskevaesvrvlyGasvtaaedaelaaqldvdGvLlasavlka 227
                                                        l+  + e a+ +r+lyG+s++a+++a+l aq+d+dG L+++a+lka
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1454 192 AQLAA-ASEHADRIRLLYGGSMNAGNAAQLLAQPDIDGGLVGGASLKA 238
                                                      87775.567799***********************************8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (228 nodes)
Target sequences:                          1  (249 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.19
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources