GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natA in Acidovorax sp. GW101-3H11

Align NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized)
to candidate Ac3H11_104 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1)

Query= TCDB::Q55164
         (267 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_104
          Length = 249

 Score =  163 bits (412), Expect = 4e-45
 Identities = 94/251 (37%), Positives = 149/251 (59%), Gaps = 9/251 (3%)

Query: 18  LLLAQGLSKSFGGLRAVDHADIVVKEGSITGLIGPNGAGKTTLFNLLSNFIRPDQGEVLF 77
           +L  +G+S  FG L+AV       + G IT +IGPNGAGK++LFNL+S  IRP  G V F
Sbjct: 4   ILSVKGISVQFGALKAVQDVSFEAQPGKITAVIGPNGAGKSSLFNLISGAIRPSSGAVRF 63

Query: 78  NGDSIGQLAPHQIALRGSVRTFQVAKVLSRLTVLENMLLADQHQTGEKFLPRLINFRRVQ 137
           +G+ +   APHQ+   G  R+FQ+  +   L V EN+ LA Q     + L     FR + 
Sbjct: 64  DGEDVTGRAPHQLLRYGLSRSFQITNLFFELPVRENLRLAAQFLEQGRGL-----FRPLA 118

Query: 138 KEERANREKAMAMLESVGLGAKAQDYAGALSGGQRKLLEMARALMSNPKLILLDEPAAGV 197
           + + A   +   +LE   L  KA + AG LS G+++ LE+A +L S P+++LLDEP  G+
Sbjct: 119 RSQTA-AARVDELLEQFDLRNKADELAGYLSHGEQRRLEIAVSLASRPRMLLLDEPTQGM 177

Query: 198 NPTLIGQICEHIVNW--NRQGITFLVIEHNMDVIMTLCHHVWVLAEGRNLADGTPEQIQS 255
           +     +  E ++    +  G++ L++EH++D++M L   V V+ +G+ LA+GTP Q+++
Sbjct: 178 SHGDTRE-TESLIRGLSHNHGLSILLVEHDVDLVMQLSDFVVVMHQGQKLAEGTPAQVRA 236

Query: 256 DPRVLEAYLGD 266
           +P V  AY G+
Sbjct: 237 NPAVQAAYFGE 247


Lambda     K      H
   0.319    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 168
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 267
Length of database: 249
Length adjustment: 24
Effective length of query: 243
Effective length of database: 225
Effective search space:    54675
Effective search space used:    54675
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory