Alignments for a candidate for ech in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Acidovorax sp. GW101-3H11

Align Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate Ac3H11_2523 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

Query= SwissProt::Q08426
         (723 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_2523
          Length = 699

 Score =  507 bits (1306), Expect = e-148
 Identities = 313/717 (43%), Positives = 432/717 (60%), Gaps = 37/717 (5%)

Query: 2   AEYTRLHNALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGA 61
           AEY ++H ++A+I + NPPVN +     + I +GL +A  D  +K+IVI GA G FS GA
Sbjct: 3   AEY-KVHGSVAVITMANPPVNGLGLATRQGIVDGLGRANADAAVKSIVITGAGGAFSGGA 61

Query: 62  DIRGFSAPRTFGL-TLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQV 120
           DI+ F   ++     L  V+  I+ + KPVVAA+  +A GGGLELALGCHYRIA     +
Sbjct: 62  DIKEFGTDKSLQEPNLLSVIAAIENSAKPVVAAMHSVAMGGGLELALGCHYRIAAPGCSI 121

Query: 121 GLPEVTLGLLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLG---ILDKVVNSDP 177
            LPEV LGL+PGA GTQ LPR+ GV AAL+LI SG  + ++    +    + DK+  S  
Sbjct: 122 ALPEVKLGLIPGAGGTQRLPRVIGVEAALNLIVSGEPVKSEMIGAVPGQKLFDKMAASAE 181

Query: 178 --VEEAIRFAQRVSD-QPLESRRLCNKPIQSLPNMDSIFSEALLKMRRQHPGCLAQEACV 234
               EA+ FAQ V+D +PL   R  N P +     D+ F  A   ++       A   CV
Sbjct: 182 SLAAEALAFAQSVADARPLPLVR--NFPCKHSEG-DAYFQFARNMVKGMAKNFPAPAKCV 238

Query: 235 RAVQAAVQYPYEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTPSGASWKTASA 294
            AV+AA +  +  G+  E E+F+ L+ + ++RAL++ F AER A+K   P  AS      
Sbjct: 239 DAVEAATKKKFAEGMLVEREIFINLMWTPESRALRHLFMAERAASK--IPDVAS--DTPK 294

Query: 295 RPVSSVGVVGLGTMGRGIVISFARARIPVIAVDSDKNQL----ATANKMITSVLEKEASK 350
           R +  VGV+G GTMG GI ++F  A IPV  ++  +  L    AT  K   + ++K   K
Sbjct: 295 RDIKLVGVIGAGTMGGGISMNFLNAGIPVKILEMKQEALDRGVATIKKNYEAQVKK--GK 352

Query: 351 MQQSGHPWSGPKPRLTSSVKELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNT 410
           ++Q  +         T S  +    DL+IEAVFEEM +K+ VF +L AV KP A L +NT
Sbjct: 353 LKQDKYDQRMALLSTTLSYDDFKDCDLIIEAVFEEMGVKEAVFKQLDAVAKPGAILASNT 412

Query: 411 SALDVDEIASSTDRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIG 470
           S LDVD+IA+ T RP  V+G HFFSPA+VMKLLEV+  + ++   +ATVM + KKIKK  
Sbjct: 413 STLDVDKIAAFTKRPQDVVGMHFFSPANVMKLLEVVRGKETAKDVLATVMAIGKKIKKTS 472

Query: 471 VVVGNCFGFVGNRMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVG 530
           VV G C GF+GNRM+  Y  QA FLL+EG  P++VD+ +E+FGF MGPFR+ DLAG D+G
Sbjct: 473 VVSGVCDGFIGNRMIEQYSRQAGFLLDEGCTPQQVDKAVEKFGFAMGPFRMGDLAGNDIG 532

Query: 531 WKSRKGQGLTGPTLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIH 590
           W  RK + +            +R + +Y    D LCELGRFGQKTG GWY Y     R  
Sbjct: 533 WAIRKRRSV------------ERADMKYSRTADKLCELGRFGQKTGAGWYDYQAG-KRDA 579

Query: 591 KPDPWLSKFLSRYRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVY 650
            P   ++K +  +RK   I PR IS +EI++R +++L+NE   IL +GIA+    ID+VY
Sbjct: 580 IPSDLVNKMIEDHRKELGITPRKISDEEIVQRLVFALVNEGAHILEDGIASKSGDIDMVY 639

Query: 651 LHGYGWPRHKGGPMFYASTVGLPTVLEKLQKYYRQNP--DIPQLEPSDYLKKLASQG 705
           L GYG+P H+GGPM YAS VGL  V++ + ++ R NP  D    +P+  L KLA++G
Sbjct: 640 LTGYGFPIHRGGPMHYASEVGLFNVVQAMDRFAR-NPLDDAAFWKPAPLLAKLAAEG 695


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1060
Number of extensions: 52
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 723
Length of database: 699
Length adjustment: 39
Effective length of query: 684
Effective length of database: 660
Effective search space:   451440
Effective search space used:   451440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources