Align Beta-ketothiolase BktB; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; EC 2.3.1.16; EC 2.3.1.9 (characterized)
to candidate Ac3H11_178 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)
Query= SwissProt::Q0KBP1 (394 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_178 Length = 394 Score = 608 bits (1567), Expect = e-178 Identities = 305/394 (77%), Positives = 338/394 (85%) Query: 1 MTREVVVVSGVRTAIGTFGGSLKDVAPAELGALVVREALARAQVSGDDVGHVVFGNVIQT 60 MTREVVVVS VRTAIGTFGGSLKD+AP +LGALVV+E+LARA V G DVGHVVFG+V+ T Sbjct: 1 MTREVVVVSAVRTAIGTFGGSLKDIAPTDLGALVVKESLARASVEGKDVGHVVFGHVVNT 60 Query: 61 EPRDMYLGRVAAVNGGVTINAPALTVNRLCGSGLQAIVSAAQTILLGDTDVAIGGGAESM 120 EP+DMYL RVAA+NGG PA VNRLCGSGLQAIVSAAQ I LGD DV IG GAE M Sbjct: 61 EPKDMYLSRVAAINGGCAEGTPAFNVNRLCGSGLQAIVSAAQAIQLGDADVTIGAGAEVM 120 Query: 121 SRAPYLAPAARWGARMGDAGLVDMMLGALHDPFHRIHMGVTAENVAKEYDISRAQQDEAA 180 SRAP+ + RWGARMGD +VDMM+GALHDPFH IHMGVTAEN+A ++ ISR QD+ A Sbjct: 121 SRAPFASLNMRWGARMGDTKMVDMMIGALHDPFHTIHMGVTAENIAAKWGISREDQDKLA 180 Query: 181 LESHRRASAAIKAGYFKDQIVPVVSKGRKGDVTFDTDEHVRHDATIDDMTKLRPVFVKEN 240 +ESH RA A AGYFKDQIVPV K +KGDV + TDEH R AT+DD KL+PVFVKEN Sbjct: 181 VESHNRAERATAAGYFKDQIVPVTLKSKKGDVQYATDEHFRPGATLDDFAKLKPVFVKEN 240 Query: 241 GTVTAGNASGLNDAAAAVVMMERAEAERRGLKPLARLVSYGHAGVDPKAMGIGPVPATKI 300 GTVTAGNASG+NDAAAAVV+M+ A A+ RG KPLARLV+Y HAGVDPK MGIGPVPAT++ Sbjct: 241 GTVTAGNASGINDAAAAVVLMDAAAAKARGAKPLARLVAYAHAGVDPKYMGIGPVPATQL 300 Query: 301 ALERAGLQVSDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALIT 360 AL++AGL V+DLDVIEANEAFAAQACAVTK LGLDPAKVNPNGSGISLGHPIGATGALIT Sbjct: 301 ALKKAGLTVADLDVIEANEAFAAQACAVTKDLGLDPAKVNPNGSGISLGHPIGATGALIT 360 Query: 361 VKALHELNRVQGRYALVTMCIGGGQGIAAIFERI 394 VKA+HEL RVQGRYALVTMCIGGGQGIAAIFER+ Sbjct: 361 VKAIHELQRVQGRYALVTMCIGGGQGIAAIFERL 394 Lambda K H 0.318 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 569 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 394 Length of database: 394 Length adjustment: 31 Effective length of query: 363 Effective length of database: 363 Effective search space: 131769 Effective search space used: 131769 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate Ac3H11_178 (3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9))
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.1776162.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9e-141 455.2 8.5 1e-140 455.0 8.5 1.0 1 lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 3-ketoacyl-CoA thiolase (EC 2.3. Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 455.0 8.5 1e-140 1e-140 1 385 [] 7 392 .. 7 392 .. 0.99 Alignments for each domain: == domain 1 score: 455.0 bits; conditional E-value: 1e-140 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageq.aniaR 62 +v+avRt+ig++ggslk++ + dL+a v+ke l+ra ++ +++ +v++G+v+++ + + + R lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 7 VVSAVRTAIGTFGGSLKDIAPTDLGALVVKESLARASVEGKDVGHVVFGHVVNTEPKdMYLSR 69 79***************************************************99998***** PP TIGR01930 63 eaalaaglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslr 125 aa++ g+ e +pa++vnr+C+SglqA+ +aaq+i+ G+adv + +G+E mSr+p+ + + lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 70 VAAINGGCAEGTPAFNVNRLCGSGLQAIVSAAQAIQLGDADVTIGAGAEVMSRAPFASLNM-- 130 *******************************************************998886.. PP TIGR01930 126 reslklgkakledqllkdl..vktklsmgetAenlakkygisReeqDeyalrShqkaakAiee 186 r+++++g++k+ d+++ l + + ++mg+tAen+a+k+gisRe+qD++a++Sh++a++A+++ lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 131 RWGARMGDTKMVDMMIGALhdPFHTIHMGVTAENIAAKWGISREDQDKLAVESHNRAERATAA 193 8************9999989999**************************************** PP TIGR01930 187 gkfkdeivpvevkgk..kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGA 247 g+fkd+ivpv++k k ++++++De+ rp+ tl++ akLkp+f +++g tvtAgN+s++nD+A lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 194 GYFKDQIVPVTLKSKkgDVQYATDEHFRPGATLDDFAKLKPVFVKENG-TVTAGNASGINDAA 255 *************99999999***************************.6************* PP TIGR01930 248 aalllmseevakelgltplarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEin 310 aa++lm ++ak+ g +plar+v++a+agvdp++mg+gpvpA++ aLkkagl++ d+d++E n lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 256 AAVVLMDAAAAKARGAKPLARLVAYAHAGVDPKYMGIGPVPATQLALKKAGLTVADLDVIEAN 318 *************************************************************** PP TIGR01930 311 EAFAaqvlavekelgsldlekvNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlC 373 EAFAaq+ av+k+lg ld++kvN nG+ i+lGHP+Ga+Ga i+++ ++eL++ +++y+l+t+C lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 319 EAFAAQACAVTKDLG-LDPAKVNPNGSGISLGHPIGATGALITVKAIHELQRVQGRYALVTMC 380 ***************.99********************************************* PP TIGR01930 374 vggGqGaAvile 385 +ggGqG+A+i+e lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_178 381 IGGGQGIAAIFE 392 **********97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (394 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 27.13 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory