Align Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 (characterized)
to candidate Ac3H11_2303 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)
Query= SwissProt::P14611 (393 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_2303 Length = 392 Score = 560 bits (1444), Expect = e-164 Identities = 281/392 (71%), Positives = 330/392 (84%) Query: 1 MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALERAGVKPEQVSEVIMGQVLTAG 60 M D+VIVSAARTAVGKFGG+LAK PA ELGA+VI+ A+ RAG+ +Q+ EVIMGQVL AG Sbjct: 1 MEDIVIVSAARTAVGKFGGALAKTPASELGAIVIREAIARAGLSSDQIGEVIMGQVLAAG 60 Query: 61 SGQNPARQAAIKAGLPAMVPAMTINKVCGSGLKAVMLAANAIMAGDAEIVVAGGQENMSA 120 GQNPARQA++KAG+ PA+TIN VCGSGLKAVMLAA A+ GD++IVVAGGQENMS Sbjct: 61 VGQNPARQASMKAGVAKETPALTINAVCGSGLKAVMLAAQAVAWGDSDIVVAGGQENMSL 120 Query: 121 APHVLPGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGITAENVAKEYGITREAQDEFAV 180 APHVL GSRDG RMGD K+VD+MIVDGLWDVYNQYHMGITAENVAK YGITR+ QD A+ Sbjct: 121 APHVLNGSRDGQRMGDWKMVDSMIVDGLWDVYNQYHMGITAENVAKAYGITRDMQDALAL 180 Query: 181 GSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQGATLDSMSGLKPAFDKAG 240 GSQ KA AAQ AGKF +EIV V + Q+KGDP+ F DE++ + ++++GL+PAFDKAG Sbjct: 181 GSQQKAAAAQDAGKFADEIVGVSLAQKKGDPILFNADEYLNRKTNAEALAGLRPAFDKAG 240 Query: 241 TVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYANAGVDPKVMGMGPVPASKRA 300 +VTA NASGLNDGAAAVVVMSA KA LGL PLA I ++ +G+DP MGMGPVPAS++A Sbjct: 241 SVTAGNASGLNDGAAAVVVMSAKKAAALGLKPLARIAAFGTSGLDPATMGMGPVPASRKA 300 Query: 301 LSRAEWTPQDLDLMEINEAFAAQALAVHQQMGWDTSKVNVNGGAIAIGHPIGASGCRILV 360 L RA W D+DL E+NEAFAAQA AV++++ D +KVNVNGGAIAIGHPIGASGCRILV Sbjct: 301 LQRAGWNAADVDLFELNEAFAAQACAVNKELAIDPAKVNVNGGAIAIGHPIGASGCRILV 360 Query: 361 TLLHEMKRRDAKKGLASLCIGGGMGVALAVER 392 TLLHEM+RRDAKKGLA+LCIGGGMGV+LA+ER Sbjct: 361 TLLHEMQRRDAKKGLAALCIGGGMGVSLALER 392 Lambda K H 0.315 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 532 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 393 Length of database: 392 Length adjustment: 31 Effective length of query: 362 Effective length of database: 361 Effective search space: 130682 Effective search space used: 130682 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate Ac3H11_2303 (3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9))
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.1117.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.4e-145 469.1 14.7 6.1e-145 468.9 14.7 1.0 1 lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 3-ketoacyl-CoA thiolase (EC 2.3. Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 468.9 14.7 6.1e-145 6.1e-145 1 385 [] 6 391 .. 6 391 .. 0.99 Alignments for each domain: == domain 1 score: 468.9 bits; conditional E-value: 6.1e-145 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaR 62 iv+a+Rt++gk+gg+l++++a++L+a vi+e+++ragl+ ++i evi+G+vl ag ++n+aR lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 6 IVSAARTAVGKFGGALAKTPASELGAIVIREAIARAGLSSDQIGEVIMGQVLAAGVGQNPAR 67 8************************************************************* PP TIGR01930 63 eaalaaglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkasl 124 +a ++ag+ +++palt+n vC+Sgl+Av+laaq+++ G+ d+vvaGG E+mS +p++l+ s lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 68 QASMKAGVAKETPALTINAVCGSGLKAVMLAAQAVAWGDSDIVVAGGQENMSLAPHVLNGS- 128 ***********************************************************98. PP TIGR01930 125 rreslklgkakledqllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakA 183 r++ ++g+ k+ d+++ d+ v+++++mg+tAen+ak ygi+R++qD++al S+qkaa+A lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 129 -RDGQRMGDWKMVDSMIVDGlwdVYNQYHMGITAENVAKAYGITRDMQDALALGSQQKAAAA 189 .8*****************9***99************************************* PP TIGR01930 184 ieegkfkdeivpvevkgk...kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssq 242 +++gkf+deiv v++ +k + ++ De + ++t++e+La+L+paf++ +gs vtAgN+s+ lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 190 QDAGKFADEIVGVSLAQKkgdPILFNADEYLNRKTNAEALAGLRPAFDK-AGS-VTAGNASG 249 ***************99999999************************95.9*7.******** PP TIGR01930 243 lnDGAaalllmseevakelgltplarivsaavagvdpeemglgpvpAiekaLkkaglsisdi 304 lnDGAaa+++ms ++a++lgl+plari +++++g+dp++mg+gpvpA++kaL++ag+++ d+ lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 250 LNDGAAAVVVMSAKKAAALGLKPLARIAAFGTSGLDPATMGMGPVPASRKALQRAGWNAADV 311 ************************************************************** PP TIGR01930 305 dlvEinEAFAaqvlavekelgsldlekvNvnGGAiAlGHPlGasGarivltllkeLkergkk 366 dl+E+nEAFAaq+ av+kel+ +d++kvNvnGGAiA+GHP+GasG+ri++tll+e+++r++k lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 312 DLFELNEAFAAQACAVNKELA-IDPAKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRRDAK 372 *********************.88************************************** PP TIGR01930 367 yGlatlCvggGqGaAvile 385 Gla+lC+ggG+G+++ le lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2303 373 KGLAALCIGGGMGVSLALE 391 **************99876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (392 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.07 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory