GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mglC in Acidovorax sp. GW101-3H11

Align Putative beta-xyloside ABC transporter, permease component, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate Ac3H11_3036 Fructose ABC transporter, permease component FrcC

Query= TCDB::G4FGN4
         (313 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_3036
          Length = 319

 Score =  226 bits (577), Expect = 4e-64
 Identities = 120/301 (39%), Positives = 179/301 (59%), Gaps = 3/301 (0%)

Query: 12  GIFLILIAIVVFLGVTTREFLTVENIFTVILNVSFIAIMSFGMTMVIITSGIDLSVGSIL 71
           G F+ LI    F    +  FL+ +N   ++  V  +A+++ G T+VI+T+GIDLS G ++
Sbjct: 15  GPFIALILACAFFATQSERFLSAQNFALILQQVMVVAVIAIGQTLVILTAGIDLSCGMVM 74

Query: 72  GAASVVMGLLMDEKGLSPFLSVVIGLAVGVGFGLANGLLITKARLAPFISTLGMLSVGRG 131
               +VM  +  + GLS  +++  G+AV + FGL NGLL+TK +L PFI TLG L++   
Sbjct: 75  ALGGIVMTKMAADYGLSAPVAIACGMAVTMLFGLINGLLVTKIKLPPFIVTLGTLNIAFA 134

Query: 132 LAYVMSGGWPISPFPESFTVHGQGM-VGPVPVP--VIYMAVIGVIAHIFLKYTVTGRRIY 188
              + SG   I+  P   T  G    +G   +    + M  + ++    L+ T  GR +Y
Sbjct: 135 ATQLYSGAQTITDIPAGMTALGNTFQLGQTAIVWGAVLMLALYLVTWFALRETAPGRHVY 194

Query: 189 AIGGNMEASKLVGIKTDRILILVYTINGFLAAFAGFLLTAWLGVAQPNAGQGYELDVIAA 248
           A+G + EA++L GI TD++L+ VY + G     A  L  A  G   PNAGQ   LD I+A
Sbjct: 195 AVGNSPEATRLTGIATDKVLLGVYVLAGLFYGIASLLSVARTGAGDPNAGQTENLDAISA 254

Query: 249 TVIGGTSLSGGEGTILGAFLGAVIMGVLRNGMILLGVSSFWQQVVIGIVIIIAIAIDQIR 308
            V+GGTSL GG G ILG  +GA+I+GV RNG+ L+GVSS +Q +V GI++I+A+A DQ+ 
Sbjct: 255 VVLGGTSLFGGRGVILGTLVGALIVGVFRNGLTLMGVSSVYQILVTGILVILAVATDQLS 314

Query: 309 R 309
           R
Sbjct: 315 R 315


Lambda     K      H
   0.328    0.145    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 263
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 313
Length of database: 319
Length adjustment: 27
Effective length of query: 286
Effective length of database: 292
Effective search space:    83512
Effective search space used:    83512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory