Align Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) (characterized)
to candidate Ac3H11_1927 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)
Query= reanno::pseudo1_N1B4:Pf1N1B4_3984 (651 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_1927 Length = 670 Score = 510 bits (1314), Expect = e-149 Identities = 308/681 (45%), Positives = 401/681 (58%), Gaps = 58/681 (8%) Query: 9 LLVANRGEIACRVMRTAKALGLTTVAVHSATDRDARHSREADIRVDLGGSKAADSYLQID 68 +L+ANR EIA R++ TA +G+ TVAV+S D A H REA V LGG+ +AD+YL+ D Sbjct: 4 ILIANRSEIARRIIHTAHRMGIETVAVYSDPDASALHVREATQAVALGGAASADTYLRTD 63 Query: 69 KLIAAAKASGAQAIHPGYGFLSENAGFARAIEAAGLIFLGPPASAIDAMGSKSAAKALME 128 KL+AAA+A+GA A+HPGYGFLSENA FA+A+ AGL ++GPP +AI A+GSK+ AKAL Sbjct: 64 KLLAAARATGADAVHPGYGFLSENADFAQAVVDAGLTWIGPPPAAIRALGSKAGAKALAV 123 Query: 129 TAGVPLVPGYHGEAQDLETFRDACERIGYPVLLKATAGGGGKGMKVVEDVSQLAEALASA 188 VP +PGY G+ Q E F RIG P+++KA AGGGG+GM++V D++QL ALASA Sbjct: 124 AHCVPCLPGYAGDDQSDERFAAEAARIGTPLMVKAVAGGGGRGMRLVTDLAQLPAALASA 183 Query: 189 QREALSSFGDSRMLVEKYLLKPRHVEIQVFADQHGNCLYLNERDCSIQRRHQKVVEEAPA 248 + EAL+ FG +L+E+ LL+PRHVE+Q+FAD HG C++L ERDCS+QRRHQK++EEAP+ Sbjct: 184 RSEALAGFGCGDLLIERALLQPRHVEVQIFADAHGACIHLGERDCSVQRRHQKIIEEAPS 243 Query: 249 PGLSPELRRAMGEAAVRSAQAIGYVGAGTVEFLLDARGEFFFMEMNTRLQVEHPVTEAIT 308 P + LR MG AV AQA GYVGAGTVEFLLD +F+ MEMNTRLQVEHPVTEA+T Sbjct: 244 PAVDAALRERMGACAVALAQAAGYVGAGTVEFLLDG-PDFYLMEMNTRLQVEHPVTEALT 302 Query: 309 GLDLVAWQIRVARGEALPMTQDQVPLIGHAIEVRLYAEDPGNDFLPATGRLALYR----- 363 GLDLV WQIRVARGE LP+TQDQV L GHAIEVRL AED F P TGR+ + Sbjct: 303 GLDLVEWQIRVARGEPLPLTQDQVHLQGHAIEVRLCAEDA--HFRPHTGRVLQFSAPPAT 360 Query: 364 --ESAAGPGRRVDSGVEEGDEISPFYDPMLGKLIAWGEDREQARLRLLSMLDEFAIGGLK 421 E AA R D +EEG E++P YD MLGKLI R +A L+ L + GL Sbjct: 361 AFERAAPGALRFDHALEEGAEVTPHYDAMLGKLIVHAPTRAEAIAALVRALHSTRVLGLP 420 Query: 422 TNINFLRRIIGHPAFAAAELDTGFIPRYQEQLLPAPSDLSDEFWQAAAQAFAQSQSSTTR 481 TN FL + HP F A F+ + QL S++ + +A A S +S+ Sbjct: 421 TNRAFLAACLQHPVFGAGHALVPFLAEHAAQLQGLLSNIELKVLVQSAVAVIFSSNSSGA 480 Query: 482 ADDLSSPWGIGNGFRAGLPTEITLHLSCEEQDRALTLGDADAHTAQLKGEYL-LTEHNGL 540 A A LP + L Q + + L + +L E+ T H L Sbjct: 481 AS-------------AQLPCALARPLRLRHQGQVVPLAVRELGAGRLWVEHAGGTTHLQL 527 Query: 541 RRQHRAIRRGDTLYLQWDGELRRIE---------------------------SYDPISAV 573 Q + R T+ GE+ + S++P + Sbjct: 528 APQAAGMVRITTMQTGAVGEVHHADHTVRAVPVGPRRWHWQTGGVDGWVEDASWEPAARA 587 Query: 574 EASHSHQGGLT---APMNGSIVRVLVEAGQTVEAGAQLVVLEAMKMEHSIRAPHAGIIKA 630 A+ GG T AP NG +V + V AGQ + AG +VV+E+MK+EHS+ +P A + Sbjct: 588 GAA----GGATELRAPFNGKVVALPVAAGQALSAGDTVVVIESMKLEHSLASPVAATVAE 643 Query: 631 LYCQEGEMVSEGSALVELEHA 651 L G+ VS G L A Sbjct: 644 LLVAPGQQVSPGQVLARFAPA 664 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1139 Number of extensions: 46 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 651 Length of database: 670 Length adjustment: 38 Effective length of query: 613 Effective length of database: 632 Effective search space: 387416 Effective search space used: 387416 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory