Aligments for a candidate for lysDH in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for lysDH in Acidovorax sp. GW101-3H11

Align lysine 6-dehydrogenase (EC 1.4.1.18) (characterized)
to candidate Ac3H11_2284 putative dehydrogenase

Query= BRENDA::Q3S559
         (368 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_2284
          Length = 369

 Score =  484 bits (1246), Expect = e-141
 Identities = 252/362 (69%), Positives = 284/362 (78%)

Query: 4   TQHAITVLGAGKIGFAIALLLQRTGDYAVCVADQDPSRLDAVAALGCQTAQIDNDAALEA 63
           T+H +T+LGAG IGFA+ALLLQ+ GDY + +AD+DP+RL  VAALG  T  + +D +L++
Sbjct: 7   TRHTVTILGAGHIGFAMALLLQQAGDYDILMADRDPARLAEVAALGVATRLVGDDTSLQS 66

Query: 64  AIAGRHAVLNALPFHRAVAVAGLCARLGVHYFDLTEDVASTHAIHALGRDARAVLMPQCG 123
           AIAGR AVLNALPFHRAV VA LCA  GVHYFDLTEDVAST AI AL   AR+VLMPQCG
Sbjct: 67  AIAGRFAVLNALPFHRAVPVATLCAAAGVHYFDLTEDVASTQAIRALAAKARSVLMPQCG 126

Query: 124 LAPGFIGIVGNDLARRFDTLLDLRMRVGGLPRYPTNALRYNLYLEHRGADQRVLQSMRGA 183
           LAPGFIGIVGNDLARRFDTL  LRMRVG LPRYP  ALRYNL     G            
Sbjct: 127 LAPGFIGIVGNDLARRFDTLHTLRMRVGALPRYPQGALRYNLTWSTEGLINEYCNPCEAI 186

Query: 184 VDGELVKVPPMEGYETFTLDGVEYEAFNTSGGLGTLPQTLLGKARNVDYKSVRYPGHCAI 243
           VDG    VP +EG ETF LDGVEYEAFNTSGGLGTL +TL GKAR VDY+S+RYPGH AI
Sbjct: 187 VDGVRTTVPALEGLETFALDGVEYEAFNTSGGLGTLTETLAGKARQVDYQSIRYPGHNAI 246

Query: 244 MKLLLNDLRLRERRELLQDILESAIPATGQDVIVILATASGYRGGRLLQEAYSAHIHGDT 303
           +KLLLNDLRLR+RR+LL+DILESAIP T QDVIVI ATA+G RGGRL+QE+YSA I G  
Sbjct: 247 LKLLLNDLRLRDRRDLLKDILESAIPTTDQDVIVIFATAAGLRGGRLVQESYSARIVGTE 306

Query: 304 VDGHALSAIQLSTAAGICTALDLVVEGALPQRGFVGQESIPLDALLANRHGRIYAGQRLD 363
           + GH LSAIQL+TAAGICTALDLV +G LPQRGFVGQE++ L   LANR G  YAG+   
Sbjct: 307 LAGHRLSAIQLTTAAGICTALDLVAQGRLPQRGFVGQEAVRLGDFLANRFGVAYAGEGAL 366

Query: 364 AL 365
           AL
Sbjct: 367 AL 368


Lambda     K      H
   0.323    0.139    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 441
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 368
Length of database: 369
Length adjustment: 30
Effective length of query: 338
Effective length of database: 339
Effective search space:   114582
Effective search space used:   114582
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources