GapMind for catabolism of small carbon sources

 

Aligments for a candidate for patD in Acidovorax sp. GW101-3H11

Align aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized)
to candidate Ac3H11_1480 Aldehyde dehydrogenase B (EC 1.2.1.22)

Query= BRENDA::P77674
         (474 letters)



>lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1480 Aldehyde
           dehydrogenase B (EC 1.2.1.22)
          Length = 486

 Score =  312 bits (799), Expect = 2e-89
 Identities = 178/472 (37%), Positives = 264/472 (55%), Gaps = 4/472 (0%)

Query: 1   MQHKLLINGELVSGE-GEKQPVYNPATGDVLLEIAEASAEQVDAAVRAADAAFAEWGQTT 59
           ++ +  I+G+ V  + G    V NPA G ++  I  A A Q   A+ AAD AF  W   T
Sbjct: 12  LKARCYIDGQWVDADNGATLAVNNPANGALIGTIPNAGAAQTQTAIAAADRAFGPWKDRT 71

Query: 60  PKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIPAIVDVFRFFAGAARCLN 119
            + RA  L +  +++ ++ +  A + +   GKPL  A   EI        +FA  AR + 
Sbjct: 72  AEDRARILRRWFELMLQHQEDLALIMTSEQGKPLAEA-RGEIAYAASYIEWFAEEARRIY 130

Query: 120 GLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLAPALAAGNCVVLKPSEITPL 179
           G            ++ R+P+GV A+I PWN+P  M   K+APALAAG  +++KP+  TPL
Sbjct: 131 GEVIPSPWLDKRIVVTREPVGVCAAITPWNFPAAMITRKVAPALAAGCTIIVKPATQTPL 190

Query: 180 TALKLAELAKDI-FPAGVINILFGRGKTVGDPLTGHPKVRMVSLTGSIATGEHIISHTAS 238
           +AL +AELA     PAGV +++ G  + +G  LT  P VR ++ TGS   G  + +  A 
Sbjct: 191 SALAMAELAARAGVPAGVFSVITGDARPIGAELTASPVVRKLTFTGSTEIGRVLAAQCAP 250

Query: 239 SIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNAGQDCTAACRIYAQKGIYDTLVE 298
           ++K+  +ELGG AP IVF+DAD++A V G     Y N GQ C  A R+  Q G+YD   E
Sbjct: 251 TLKKMSLELGGNAPFIVFEDADLDAAVAGAMASKYRNTGQTCVCANRLLVQDGVYDAFAE 310

Query: 299 KLGAAVATLKSGAPDDESTELGPLSSLAHLERVGKAVEEAKATGHIKVITGGEKRKGNGY 358
           KL   VA LK G   +E  E GPL   A L +V   V +AKA G  +V+TGG +    G 
Sbjct: 311 KLARTVAALKVGHGLEEGVEQGPLIDEASLTKVEALVADAKARG-ARVVTGGRRHALGGT 369

Query: 359 YYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNEEQVVNWANDSQYGLASSVWTKDVGRA 418
           +Y PT+LA    D  + ++E+FGPV  +  F  E + +  AND+++GLA+  +++DVGR 
Sbjct: 370 FYEPTILADITPDMRMAREEIFGPVAPLFRFHTEAEAIQMANDTEFGLAAYFYSRDVGRV 429

Query: 419 HRVSARLQYGCTWVNTHFMLVSEMPHGGQKLSGYGKDMSLYGLEDYTVVRHV 470
            RVS  LQYG   +N   +  +  P GG K SG G++ S++G+E+Y   +++
Sbjct: 430 WRVSGALQYGMVGINAGVISTAVAPFGGVKQSGMGREGSVHGIEEYVDTKYL 481


Lambda     K      H
   0.317    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 546
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 474
Length of database: 486
Length adjustment: 34
Effective length of query: 440
Effective length of database: 452
Effective search space:   198880
Effective search space used:   198880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory