Alignments for a candidate for iatA in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for iatA in Acidovorax sp. GW101-3H11

Align Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized)
to candidate Ac3H11_609 L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)

Query= TCDB::B8H229
         (515 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_609
          Length = 505

 Score =  363 bits (931), Expect = e-104
 Identities = 208/500 (41%), Positives = 314/500 (62%), Gaps = 10/500 (2%)

Query: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAA--HAADAGTV 60
           LL++  + K+FPGV AL+QV+L V  GE+HA++GENGAGKSTL+K+LS    H + +G +
Sbjct: 2   LLEMRNIRKTFPGVVALNQVNLQVQAGEIHAIVGENGAGKSTLMKVLSGVYPHGSYSGQI 61

Query: 61  TFAGQVLDPRDAPLR-RQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLRA 119
            F GQ  +   A +R  + LGI  I+QE  L P LS+AEN++LG E  R G++DW    +
Sbjct: 62  LFDGQ--EREFAGIRDSEHLGIIIIHQELALVPLLSIAENIFLGNETARHGVIDWMAAHS 119

Query: 120 DAQALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRL 179
            AQALL+ +GL  +PD PV  L V +QQ+VEIAKA++   RL+I+DEPTA+L+  +   L
Sbjct: 120 RAQALLHKVGLGESPDTPVGQLGVGKQQLVEIAKALSRKVRLLILDEPTASLNENDSQAL 179

Query: 180 HAIIAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVAD--MVRLMV 237
             ++  LKA+ ++ I +SH+L E+  + D  TV+RDG  V   D  +  V++  +++ MV
Sbjct: 180 LDLLLELKAQGITCILISHKLNEISRVADAITVLRDGSTVQMLDCREGPVSEDRVIQAMV 239

Query: 238 GRHVEFERRKRRRPPGAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAG 297
           GR +     +R+   G +V +V       P+ S   +L+ +    R GEIVG+AGL+GAG
Sbjct: 240 GREMSDRYPQRQPQVGEIVFEVRNWRAHHPQRSDREHLKGIDLNVRRGEIVGIAGLMGAG 299

Query: 298 RTDLARLIFGAD--PIAAGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIR 355
           RT+LA  IFG       +G V +  +P+ + +   A+  G+  V EDRK  G  L+  I+
Sbjct: 300 RTELAMSIFGRSWGQRISGEVRLHGQPIDVSTVEKAVSHGLAYVTEDRKGNGLVLNEDIQ 359

Query: 356 RNLSLPSLKALSALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGR 415
            N SL +L  +S     +D   E  + + YR+KLRI+ +  +     LSGGNQQKV+L +
Sbjct: 360 FNTSLANLPGVS-FASVIDSGQEHRVAQDYREKLRIRCSGVDQKTLNLSGGNQQKVVLSK 418

Query: 416 AMALTPKVLIVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVF 475
            +  +P+VLI+DEPTRGID+GAK E++ +++ LA  G  V+VISSE+ E++ ++DRI V 
Sbjct: 419 WLFTSPEVLILDEPTRGIDVGAKYEIYTLIAQLAAEGKCVIVISSEMPELLGITDRIYVM 478

Query: 476 REGVIVADLDAQTATEEGLM 495
            EG  VA++    A++E +M
Sbjct: 479 NEGRFVAEMPTSEASQEKIM 498



 Score = 54.3 bits (129), Expect = 1e-11
 Identities = 56/227 (24%), Positives = 97/227 (42%), Gaps = 9/227 (3%)

Query: 19  LDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAAD--AGTVTFAGQVLDPRDAPLRR 76
           L  +DL V  GE+  + G  GAG++ L   +         +G V   GQ +D      + 
Sbjct: 277 LKGIDLNVRRGEIVGIAGLMGAGRTELAMSIFGRSWGQRISGEVRLHGQPIDVSTVE-KA 335

Query: 77  QQLGIATIYQEFN---LFPELSVAENMYLGREP--RRLGLVDWSRLRADAQALLNDLGLP 131
              G+A + ++     L     +  N  L   P      ++D  +    AQ     L + 
Sbjct: 336 VSHGLAYVTEDRKGNGLVLNEDIQFNTSLANLPGVSFASVIDSGQEHRVAQDYREKLRIR 395

Query: 132 LNP-DAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAIIAGLKARS 190
            +  D     L+   QQ V ++K +  +  ++I+DEPT  +       ++ +IA L A  
Sbjct: 396 CSGVDQKTLNLSGGNQQKVVLSKWLFTSPEVLILDEPTRGIDVGAKYEIYTLIAQLAAEG 455

Query: 191 VSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRLMV 237
             VI +S  + E+  + DR  VM +GRFVA    ++     ++R +V
Sbjct: 456 KCVIVISSEMPELLGITDRIYVMNEGRFVAEMPTSEASQEKIMRAIV 502


Lambda     K      H
   0.320    0.136    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 640
Number of extensions: 30
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 505
Length adjustment: 34
Effective length of query: 481
Effective length of database: 471
Effective search space:   226551
Effective search space used:   226551
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources