Aligments for a candidate for bamH in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for bamH in Acidovorax sp. GW101-3H11

Align Benzoyl-CoA reductase electron transfer protein, putative (characterized, see rationale)
to candidate Ac3H11_1447 NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)

Query= uniprot:Q39TW5
         (635 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_1447
          Length = 452

 Score =  317 bits (811), Expect = 9e-91
 Identities = 168/412 (40%), Positives = 249/412 (60%), Gaps = 8/412 (1%)

Query: 150 SMDDYLAIGGYSALSKVLFQ-----MTPEDVMGEIKKSNLRGRGGGGFPAWRKWEESRNA 204
           S+ DY A GGY+AL K+L       +T + V+  +K+S LRGRGG GFP   KW     +
Sbjct: 39  SIKDYEARGGYAALRKILGTDGGEPLTQDQVIATVKESGLRGRGGAGFPTGLKWSFMPRS 98

Query: 205 PDPIKYVIVNADEGDPGAFMDRALIEGNPHSILEGLIIGAYAVGAHEGFIYVRQEYPLAV 264
               KY++ N+DEG+PG   DR +++ NPH ++EG+II AYA+G   G+ Y+  E     
Sbjct: 99  FPGQKYLVCNSDEGEPGTCKDRDILQFNPHIVIEGMIIAAYAMGISVGYNYIHGEIFQTY 158

Query: 265 ENINLAIRQASERGFVGKDILGSGFDFTVKVHMGAGAFVCGESSALMTALEGRAGEPRPK 324
           +    A+ +A   G++G  ILGS F F +    G GA++CGE +AL+ +LEG+ G+PR K
Sbjct: 159 DRFEAALEEARAAGYLGDKILGSNFSFQLHAAHGFGAYICGEETALLESLEGKKGQPRFK 218

Query: 325 YIHTAVKGVWDHPSVLNNVETWANVTQIITKGADWFTSYGTAGSTGTKIFSLVGKITNTG 384
               A  G++  P+ +NN ET+A V  II  G   +   G   + GTKIFS+ G +   G
Sbjct: 219 PPFPASFGLYGKPTTINNTETFAAVPWIIRNGGAAYLECGKPNNGGTKIFSVSGDVEKPG 278

Query: 385 LVEVPMGVTLRDIITKVGGGIPGGKKFKAVQTGGPSGGCIPEAML-DLPVDFDELTKAGS 443
             EVP+G     ++ ++ GG+  G++ KAV  GG S   +P +++ +  +D+D + KAGS
Sbjct: 279 NYEVPLGTPFAKLL-ELAGGVRKGRQLKAVIPGGSSAPVLPASIIMECTMDYDSIAKAGS 337

Query: 444 MMGSGGMIVMDEDTCMVDIARYFIDFLKDESCGKCTPCREGIRQMLAVLTRITVGKGKEG 503
           M+GSG +IVMD+   MV+       F   ESCG+CTPCREG   M  V+ RI  G+G++G
Sbjct: 338 MLGSGAVIVMDDSRSMVESLLRLSYFYSHESCGQCTPCREGTGWMWRVIDRIQHGQGRDG 397

Query: 504 DIELLEELAES-TGAALCALGKSAPNPVLSTIRYFRDEYEAHIREKKCPALS 554
           D+ LL  +A++  G  +CALG +A  PV + I++FR E+EA IR++K P  S
Sbjct: 398 DLNLLNSVADNIQGRTICALGDAAAMPVRAMIKHFRPEFEALIRQEKTPQAS 449


Lambda     K      H
   0.319    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 758
Number of extensions: 38
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 452
Length adjustment: 35
Effective length of query: 600
Effective length of database: 417
Effective search space:   250200
Effective search space used:   250200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources