Aligments for a candidate for bamH in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for bamH in Acidovorax sp. GW101-3H11

Align Benzoyl-CoA reductase electron transfer protein, putative (characterized, see rationale)
to candidate Ac3H11_3809 tungsten-containing formate dehydrogenase beta subunit

Query= uniprot:Q39TW5
         (635 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_3809
          Length = 635

 Score =  268 bits (686), Expect = 4e-76
 Identities = 175/521 (33%), Positives = 276/521 (52%), Gaps = 40/521 (7%)

Query: 28  ISVCAGAGCLASGAAEVIAAFKTELEFHGLTTEVNTKGTGCPGFCERGPIVMIYPEGICY 87
           + VC    C  +GA E++AA    L   G   +V      C G CE+ P+ +++    C 
Sbjct: 119 LRVCDSLSCSLAGARELLAALPERLRAAG-QGDVQVLAVPCVGRCEQAPVAVVHQ---C- 173

Query: 88  LKVKPEDVPEIVSHTIKEKKVVDRLLYEDPATGTRALRESDIPFYKNQQRNILSENLRLD 147
                  VP     T+ E  VV+       A   +AL+  +       +++I + +LR  
Sbjct: 174 ------PVPRATVDTVLE--VVESKPKRALARHPQALKAINFDVAALAEKSIPT-SLREV 224

Query: 148 SKSMDD---YLAIGGY-SALSKVLFQMTPEDVMGEIKKSNLRGRGGGGFPAWRKWEESRN 203
           S +  D   Y A GGY +A + V  +M  E V+  ++ S LRG GG GFPA RKW   R+
Sbjct: 225 SPAHTDLATYRAHGGYQTAAALVNGEMDAEAVLAAMEDSGLRGLGGAGFPAGRKWRIVRD 284

Query: 204 APDPIKYVIVNADEGDPGAFMDRALIEGNPHSILEGLIIGAYAVGAHEGFIYVRQEYPLA 263
            P P + + VN DEG+PG F DR  +E +PH  LEG++I A  VG    +IY+R EY   
Sbjct: 285 QPAP-RLMAVNIDEGEPGTFKDRTYLERDPHRFLEGVLIAAQVVGTEAIYIYLRDEY--- 340

Query: 264 VENINLAIRQASERGFVGKDILGSGFDFT---VKVHMGAGAFVCGESSALMTALEGRAGE 320
                + ++QA        D L +        +++  GAGA++CGE SA++ ++EG+ GE
Sbjct: 341 -HGCRVLLQQA-------LDDLRAEPPCPLPHIELRRGAGAYICGEESAMIESIEGKRGE 392

Query: 321 PRPKYIHTAVKGVWDHPSVLNNVETWANVTQIITKGADWFTSYGTAGSTGTKIFSLVGKI 380
           PR +  + A  G++  P++ +N ET   V  I+ +G  WF S+G  G  G + FS+ G++
Sbjct: 393 PRMRPPYIAQVGLFGRPTLEHNFETLYWVRDIVERGPQWFASFGRHGRKGLRSFSVSGRV 452

Query: 381 TNTGLVEVPMGVTLRDIITKVGGGIPGGKKFKAVQTGGPSGGCIPEAMLDLPVDFDELTK 440
            + G+   P G+T++++I +  GG+  G +  A   GG SGG +P ++  +P+DFD L  
Sbjct: 453 KHPGVKLAPAGITVQELIDEYCGGLLDGHQLYAYLPGGASGGILPASLNQIPLDFDTLQP 512

Query: 441 AGSMMGSGGMIVMDEDTCMVDIARYFIDFLKDESCGKCTPCREGIRQMLAVLTRITVGKG 500
            G  +GS  +IV+ +     D A   + F + ESCG+CTPCR G  +        T+ + 
Sbjct: 513 YGCFIGSAAVIVLSQHDRARDAALNVMRFFEHESCGQCTPCRVGTAK------AATLMEA 566

Query: 501 KEGDIELLEELAESTG-AALCALGKSAPNPVLSTIRYFRDE 540
            + D +LL++LA+  G A++C LG++APNP+    +YF  E
Sbjct: 567 PQWDGDLLDDLAQVMGDASICGLGQAAPNPIRCIHKYFPHE 607


Lambda     K      H
   0.319    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 946
Number of extensions: 49
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 635
Length of database: 635
Length adjustment: 38
Effective length of query: 597
Effective length of database: 597
Effective search space:   356409
Effective search space used:   356409
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources