Align Enoyl-CoA hydratase; EC 4.2.1.17 (characterized, see rationale)
to candidate Ac3H11_4989 Enoyl-CoA hydratase (EC 4.2.1.17)
Query= uniprot:A0A2Z5MCI7 (262 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_4989 Length = 266 Score = 110 bits (274), Expect = 4e-29 Identities = 80/238 (33%), Positives = 117/238 (49%), Gaps = 9/238 (3%) Query: 19 LTLSNPGARNALHPDMYAAGIEALDSVERDPSIRAVVITGADNFFCAGGNLNRLLENRAK 78 L + P A NA+ M A + A+ +V DP++RAVV+ G F AGG+L L Sbjct: 24 LRFNRPEALNAIDVPMANAFLAAVQTVAADPAVRAVVLCGNGRGFMAGGDLATL----RA 79 Query: 79 DPSVQAQSIDLLAEWISALRLSSK---PVIAAVDGAAAGAGFSLALACDLIVAADDAKFV 135 DP A ID+L +AL L ++ PV+A V G AAGAG SL L D ++AA+ + Sbjct: 80 DPVQSA--IDILTPLNAALLLLAQMNAPVVAQVHGVAAGAGLSLLLMADYVIAAEGTRLN 137 Query: 136 MSYARVGLTPDGGGSWFLAQALPRQLATEVLIEGKPIGAARLHELGVVNKLTKPGTARDA 195 ++Y +G + D G SW L + + + A E+ + G A LG+VN++ A Sbjct: 138 LAYINLGTSCDVGASWALPRIVGVRQALEIALLGDAFTADDALRLGLVNRVVPAAELDSA 197 Query: 196 AVAWADELGKISPNSVARIKTLVCAAGTQPLSEHLVAERDNFVASLHHREGLEGISAF 253 A A L + +K L+ A+ L E L AE+D FV + G+ AF Sbjct: 198 TAALAQRLASGPTLAYGAMKRLMRASMDHTLPEQLAAEKDAFVHCAGTEDFRAGVEAF 255 Lambda K H 0.317 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 149 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 262 Length of database: 266 Length adjustment: 25 Effective length of query: 237 Effective length of database: 241 Effective search space: 57117 Effective search space used: 57117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory