Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate Ac3H11_2523 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)
Query= metacyc::MONOMER-20678 (699 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_2523 Length = 699 Score = 657 bits (1696), Expect = 0.0 Identities = 359/692 (51%), Positives = 464/692 (67%), Gaps = 20/692 (2%) Query: 13 VAIVTVDSPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFG--K 70 VA++T+ +PPVN L A R+GI++ + A AD AV++IV+ AG F GADI EFG K Sbjct: 11 VAVITMANPPVNGLGLATRQGIVDGLGRANADAAVKSIVITGAGGAFSGGADIKEFGTDK 70 Query: 71 PPQPPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGL 130 Q P L VIAA+ENS KP +AA+H A+GGGLE+ALGCH+R+A + LPEVKLGL Sbjct: 71 SLQEPNLLSVIAAIENSAKPVVAAMHSVAMGGGLELALGCHYRIAAPGCSIALPEVKLGL 130 Query: 131 LPGAGGTQRLPRAVGPELAVQMIVGGSP-----IGAAEALK-HGLVEEVVENLVAGAVAF 184 +PGAGGTQRLPR +G E A+ +IV G P IGA K + E+L A A+AF Sbjct: 131 IPGAGGTQRLPRVIGVEAALNLIVSGEPVKSEMIGAVPGQKLFDKMAASAESLAAEALAF 190 Query: 185 AKKVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACADAIGAAID 244 A+ V A+ RPL +R+ K + A N V M A+ AP C DA+ AA Sbjct: 191 AQSV-ADARPLPLVRNFPCKHSEGDAYFQFARNMVKGM---AKNFPAPAKCVDAVEAATK 246 Query: 245 LPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAIIGAG 304 F EG+ ERE F+ L+ + +S+A R+ F AER A+K+ V T R + V +IGAG Sbjct: 247 KKFAEGMLVEREIFINLMWTPESRALRHLFMAERAASKIPDVASDTPKRDIKLVGVIGAG 306 Query: 305 TMGGGIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRMALIT 364 TMGGGI+M+F NAGIPV ++E +E L RG+ ++KN+EA +G L D +RMAL++ Sbjct: 307 TMGGGISMNFLNAGIPVKILEMKQEALDRGVATIKKNYEAQVKKGKLKQDKYDQRMALLS 366 Query: 365 GLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAATTKR 424 + ++ KD DLIIEAVFE M VK+ VF +DA AKPGA+LASNTS L +D+IAA TKR Sbjct: 367 TTLSYDDFKDCDLIIEAVFEEMGVKEAVFKQLDAVAKPGAILASNTSTLDVDKIAAFTKR 426 Query: 425 PQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFVGNRM 484 PQDV+GMHFFSPANVMKL E+VRG +TA D L T ++I KKI K VV GVCDGF+GNRM Sbjct: 427 PQDVVGMHFFSPANVMKLLEVVRGKETAKDVLATVMAIGKKIKKTSVVSGVCDGFIGNRM 486 Query: 485 LAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKDRGIK---- 540 + S+Q+ LL EG PQQVD V KFG MGPF MGDLAG DIGW RK R ++ Sbjct: 487 IEQYSRQAGFLLDEGCTPQQVDKAVEKFGFAMGPFRMGDLAGNDIGWAIRKRRSVERADM 546 Query: 541 --SEIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRDITDE 598 S AD LCE GRFGQKTG G+Y Y+ G R +P V +I D +LG+ R I+DE Sbjct: 547 KYSRTADKLCELGRFGQKTGAGWYDYQAGKRDAIPSDLVNKMIEDHRKELGITPRKISDE 606 Query: 599 EILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAE 658 EI++R+V+ ++NEGA ILE+ IA++ DID+V+L GYG+PI+RGGPMHYA VGL ++ + Sbjct: 607 EIVQRLVFALVNEGAHILEDGIASKSGDIDMVYLTGYGFPIHRGGPMHYASEVGLFNVVQ 666 Query: 659 RLSAYAKATNDPSL--EPAPLLARLAAEGKTF 688 + +A+ D + +PAPLLA+LAAEGK F Sbjct: 667 AMDRFARNPLDDAAFWKPAPLLAKLAAEGKAF 698 Lambda K H 0.317 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1049 Number of extensions: 52 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 699 Length of database: 699 Length adjustment: 39 Effective length of query: 660 Effective length of database: 660 Effective search space: 435600 Effective search space used: 435600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory