GapMind for catabolism of small carbon sources

 

Alignments for a candidate for paaJ1 in Acidovorax sp. GW101-3H11

Align 3-oxoadipyl-CoA thiolase; EC 2.3.1.174 (characterized, see rationale)
to candidate Ac3H11_3920 Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)

Query= uniprot:A0A2Z5MFE9
         (400 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_3920
          Length = 403

 Score =  593 bits (1529), Expect = e-174
 Identities = 301/397 (75%), Positives = 337/397 (84%)

Query: 4   AYICDAIRTPIGRYGGALKDVRADDLGAVPIKALIQRNPGVDWRAVDDVIYGCANQAGED 63
           A+ICDAIRTP GRYGGAL  VR DDLGA+PIKAL+ RNPGVDW AV DV YGCANQAGED
Sbjct: 7   AFICDAIRTPFGRYGGALSSVRTDDLGAIPIKALMDRNPGVDWAAVTDVFYGCANQAGED 66

Query: 64  NRNVARMSALLAGLPADAPGATINRLCGSGMDAVGTAARAIKAGEAQLMIAGGVESMTRA 123
           NRNVA MS+LLAGLP +  GATINRLCGSG+DAVGTAARAIKAGEA LMIAGGVESM+RA
Sbjct: 67  NRNVAHMSSLLAGLPIEVAGATINRLCGSGLDAVGTAARAIKAGEAGLMIAGGVESMSRA 126

Query: 124 PFVMGKAASAFTRQAEIHDTTIGWRFVNPLMKRQYGVDSMPETAENVAEQFGISRADQDA 183
           PFVM KA SAF+R + ++DTTIGWRFVN LMK +YGVDSMPETAENVA  F I R  QD 
Sbjct: 127 PFVMPKAESAFSRNSAVYDTTIGWRFVNKLMKEKYGVDSMPETAENVATDFKIEREAQDQ 186

Query: 184 FALASQQKAARAQRDGTLAQEIVGVEIAQKKGDAIRVTLDEHPRETSLESLARLKGVVRP 243
            AL SQ  A  A + G LA+EIV V I QKKGDAI V+ DEHPRETSLE+LA+LKGVVRP
Sbjct: 187 MALRSQLNAVAAIKAGHLAREIVPVHIPQKKGDAIIVSQDEHPRETSLEALAKLKGVVRP 246

Query: 244 DGTVTAGNASGVNDGACALLIASQQAAEQYGLRRRARVVGMATAGVEPRIMGIGPAPATQ 303
           DGTVTAGNASGVNDGACALL+A +  A +YGL+ RARVVGMA AGV PRIMG GP PATQ
Sbjct: 247 DGTVTAGNASGVNDGACALLLADEANAAKYGLKPRARVVGMAVAGVAPRIMGFGPTPATQ 306

Query: 304 KLLRQLGMTLDQLDVIELNEAFASQGLAVLRMLGLRDDDPRVNPNGGAIALGHPLGASGA 363
           K+L Q G+T+D +DVIELNEAFA+QGLAVLR LGL+DDD RVN  GGAIALGHPLGASGA
Sbjct: 307 KVLAQTGLTIDHMDVIELNEAFAAQGLAVLRALGLKDDDGRVNAWGGAIALGHPLGASGA 366

Query: 364 RLVTTALHQLERSNGRFALCTMCIGVGQGIALVIERL 400
           RLVTTA+++L    G++ALCTMCIGVGQGIA+++ER+
Sbjct: 367 RLVTTAVNRLHEHAGQYALCTMCIGVGQGIAVILERV 403


Lambda     K      H
   0.319    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 529
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 403
Length adjustment: 31
Effective length of query: 369
Effective length of database: 372
Effective search space:   137268
Effective search space used:   137268
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory