Align 3-oxoadipyl-CoA thiolase; EC 2.3.1.174 (characterized, see rationale)
to candidate Ac3H11_3920 Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)
Query= uniprot:A0A2Z5MFE9 (400 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_3920 Length = 403 Score = 593 bits (1529), Expect = e-174 Identities = 301/397 (75%), Positives = 337/397 (84%) Query: 4 AYICDAIRTPIGRYGGALKDVRADDLGAVPIKALIQRNPGVDWRAVDDVIYGCANQAGED 63 A+ICDAIRTP GRYGGAL VR DDLGA+PIKAL+ RNPGVDW AV DV YGCANQAGED Sbjct: 7 AFICDAIRTPFGRYGGALSSVRTDDLGAIPIKALMDRNPGVDWAAVTDVFYGCANQAGED 66 Query: 64 NRNVARMSALLAGLPADAPGATINRLCGSGMDAVGTAARAIKAGEAQLMIAGGVESMTRA 123 NRNVA MS+LLAGLP + GATINRLCGSG+DAVGTAARAIKAGEA LMIAGGVESM+RA Sbjct: 67 NRNVAHMSSLLAGLPIEVAGATINRLCGSGLDAVGTAARAIKAGEAGLMIAGGVESMSRA 126 Query: 124 PFVMGKAASAFTRQAEIHDTTIGWRFVNPLMKRQYGVDSMPETAENVAEQFGISRADQDA 183 PFVM KA SAF+R + ++DTTIGWRFVN LMK +YGVDSMPETAENVA F I R QD Sbjct: 127 PFVMPKAESAFSRNSAVYDTTIGWRFVNKLMKEKYGVDSMPETAENVATDFKIEREAQDQ 186 Query: 184 FALASQQKAARAQRDGTLAQEIVGVEIAQKKGDAIRVTLDEHPRETSLESLARLKGVVRP 243 AL SQ A A + G LA+EIV V I QKKGDAI V+ DEHPRETSLE+LA+LKGVVRP Sbjct: 187 MALRSQLNAVAAIKAGHLAREIVPVHIPQKKGDAIIVSQDEHPRETSLEALAKLKGVVRP 246 Query: 244 DGTVTAGNASGVNDGACALLIASQQAAEQYGLRRRARVVGMATAGVEPRIMGIGPAPATQ 303 DGTVTAGNASGVNDGACALL+A + A +YGL+ RARVVGMA AGV PRIMG GP PATQ Sbjct: 247 DGTVTAGNASGVNDGACALLLADEANAAKYGLKPRARVVGMAVAGVAPRIMGFGPTPATQ 306 Query: 304 KLLRQLGMTLDQLDVIELNEAFASQGLAVLRMLGLRDDDPRVNPNGGAIALGHPLGASGA 363 K+L Q G+T+D +DVIELNEAFA+QGLAVLR LGL+DDD RVN GGAIALGHPLGASGA Sbjct: 307 KVLAQTGLTIDHMDVIELNEAFAAQGLAVLRALGLKDDDGRVNAWGGAIALGHPLGASGA 366 Query: 364 RLVTTALHQLERSNGRFALCTMCIGVGQGIALVIERL 400 RLVTTA+++L G++ALCTMCIGVGQGIA+++ER+ Sbjct: 367 RLVTTAVNRLHEHAGQYALCTMCIGVGQGIAVILERV 403 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 529 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 403 Length adjustment: 31 Effective length of query: 369 Effective length of database: 372 Effective search space: 137268 Effective search space used: 137268 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate Ac3H11_3920 (Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-))
to HMM TIGR02430 (pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02430.hmm # target sequence database: /tmp/gapView.25136.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02430 [M=400] Accession: TIGR02430 Description: pcaF: 3-oxoadipyl-CoA thiolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-213 695.0 10.6 1.8e-213 694.8 10.6 1.0 1 lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 Acetyl-CoA acetyltransferase (EC Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thio # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 694.8 10.6 1.8e-213 1.8e-213 1 400 [] 5 403 .] 5 403 .] 1.00 Alignments for each domain: == domain 1 score: 694.8 bits; conditional E-value: 1.8e-213 TIGR02430 1 kevyivdairtpiGrygGslssvraddlaavplkallarnpsldaaaiddvilGcanqaGed 62 ++++i+dairtp+GrygG+lssvr+ddl+a+p+kal++rnp +d+aa+ dv++GcanqaGed lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 5 RQAFICDAIRTPFGRYGGALSSVRTDDLGAIPIKALMDRNPGVDWAAVTDVFYGCANQAGED 66 689*********************************************************** PP TIGR02430 63 nrnvarmaallaGlpvsvpgttvnrlcgsgldalglaaraikaGeadlviaGGvesmsrapf 124 nrnva m+ llaGlp +v+g+t+nrlcgsglda+g+aaraikaGea l+iaGGvesmsrapf lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 67 NRNVAHMSSLLAGLPIEVAGATINRLCGSGLDAVGTAARAIKAGEAGLMIAGGVESMSRAPF 128 ************************************************************** PP TIGR02430 125 vlGkadsafsrsakledttiGwrfvnpklkalyGvdsmpetaenvaeefgvsredqdafalr 186 v+ ka+safsr+ + dttiGwrfvn +k+ yGvdsmpetaenva++f+++re qd++alr lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 129 VMPKAESAFSRNSAVYDTTIGWRFVNKLMKEKYGVDSMPETAENVATDFKIEREAQDQMALR 190 ************************************************************** PP TIGR02430 187 sqqrtaaaqakGffaeeivpveikqkkGeetvvdkdehlraettlealaklkavvredgtvt 248 sq ++ aa ++G++a+eivpv+i+qkkG++ +v++deh+r et+lealaklk+vvr+dgtvt lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 191 SQLNAVAAIKAGHLAREIVPVHIPQKKGDAIIVSQDEHPR-ETSLEALAKLKGVVRPDGTVT 251 ****************************************.********************* PP TIGR02430 249 aGnasGvndGaaalllaseeavkrhgltprarilaaasaGveprvmGlgpvpavkkllarag 310 aGnasGvndGa+allla e ++++gl+prar++++a aGv+pr+mG+gp+pa++k+la++g lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 252 AGNASGVNDGACALLLADEANAAKYGLKPRARVVGMAVAGVAPRIMGFGPTPATQKVLAQTG 313 ************************************************************** PP TIGR02430 311 lsledldvielneafaaqalavlrelgladddarvnpnGGaialGhplGasGarlvltalkq 372 l+++++dvielneafaaq+lavlr+lgl+ddd rvn+ GGaialGhplGasGarlv+ta+++ lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 314 LTIDHMDVIELNEAFAAQGLAVLRALGLKDDDGRVNAWGGAIALGHPLGASGARLVTTAVNR 375 ************************************************************** PP TIGR02430 373 leksggryalatlciGvGqGialvierv 400 l+++ g+yal+t+ciGvGqGia+++erv lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3920 376 LHEHAGQYALCTMCIGVGQGIAVILERV 403 ***************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (400 nodes) Target sequences: 1 (403 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.08 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory