Alignments for a candidate for pimF in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Acidovorax sp. GW101-3H11

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate Ac3H11_1931 Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

Query= metacyc::MONOMER-20678
         (699 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_1931
          Length = 727

 Score =  252 bits (644), Expect = 4e-71
 Identities = 208/706 (29%), Positives = 329/706 (46%), Gaps = 56/706 (7%)

Query: 4   VVTRATQDQVAIVTVD--SPPVNALSAAVRRGILENVNAAVAD-----PAVQAIVLVCAG 56
           +  + T  +VA++T D  + PVN +    ++ + E      AD      A++ IVL  A 
Sbjct: 9   IPAQGTDGEVAVITFDETNSPVNTMCRQWQQELGEVTARVAADRERLGAALKGIVLASAK 68

Query: 57  RTFIAGADIT---EFGKPPQPPALNDV------IAALENSPKPTIAAIHGTALGGGLEVA 107
            +F AGAD+           P A  ++         LE    P +A ++G ALGGG EVA
Sbjct: 69  TSFFAGADLKATMRLTPADAPNAFGEIERMKKHFRTLETLGIPVVACLNGAALGGGWEVA 128

Query: 108 LGCHFRVAVKEAKL--GLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEAL 165
           L  H+R+AV + K+  GLPEV LGL+PGA G  ++ R +G   A   I+ G     AEAL
Sbjct: 129 LVAHYRIAVADPKIQFGLPEVTLGLMPGATGVTKMTRLLGLMGAQPYILEGKLFNPAEAL 188

Query: 166 KHGLVEEVV---ENLVAGAVAFAKKVLAEKRPLRRLRDDDSKL-AAAKADRSI---FTNA 218
           +  LV E+V   E L+  A+A    +      ++   D + K+     A+  I    T A
Sbjct: 189 ELQLVHELVATREQLLPAALAH---ISTHPHAVQPWDDKNYKMPGGTPANPKIAGALTVA 245

Query: 219 VAAMTKKARG-LEAPFACADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAE 277
            A + K  RG   AP A   A+     + ++  L+ E     +L+    ++     FF +
Sbjct: 246 PAMLKKTTRGRYPAPEAALAAMVEGAMVDYDTALRIESRYLARLMTGPVARNMINTFFFD 305

Query: 278 REAAKVD----GVPDGTKPR-PVSRVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLK 332
             A K       V     PR    +V I+GAG MG GIA + A+ GI   L +   E+ +
Sbjct: 306 MNAIKSGKSRPAVNGAVAPRYKPQKVGILGAGMMGAGIAWAQASRGIATVLKDVSAEKAE 365

Query: 333 RGLGIMQKNWEATAARGGLPPDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEV 392
            G     K  +A   +G +  +A    ++ IT      ++   DLIIEAVFE+  +K +V
Sbjct: 366 AGKAYSAKLTQARVDKGRMTAEAQQALLSRITPTASAADLAGCDLIIEAVFESRDLKAKV 425

Query: 393 FTAVDAHAKPGAVLASNTSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTA 452
               +    PG   ASNTS L I  +A  + RP+  +G+HFFSP + MKL EI+RG +T 
Sbjct: 426 TQEAEPQLAPGGFFASNTSTLPISGLATASSRPEKFVGIHFFSPVDKMKLVEIIRGNQTD 485

Query: 453 PDALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKF 512
            + +  A    + + KVP+VV    GF  +R       +   +L EG     ++    + 
Sbjct: 486 DETVARAFDYVQALGKVPIVVNDSRGFYTSRTFGTFVMEGAAMLGEGIPAAAIENAAMQA 545

Query: 513 GMPMGPFAMGDLAGLDIGW----RSRKDRGIKSEIADA----------LCEAGRFGQKTG 558
           G+P+GP A+ D   L +      ++R D   + +   A          + E  R G+  G
Sbjct: 546 GLPVGPLAVMDETALTLSVHVLDQTRADYATEGKTYTATPGELLVERMVKELKRPGRAGG 605

Query: 559 KGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRDITDEEILERMVYPMINEGARILEE 618
            G+Y Y  G++  +  PE++ L      K G+   + + +EI +R++Y    E AR L E
Sbjct: 606 GGFYDYPAGAKKHL-WPELKPLFE----KPGV---EWSVQEIQDRLLYRQAIETARCLAE 657

Query: 619 KIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAERLSAYA 664
            +     D ++  ++G G+P + GG M +    G++   +R    A
Sbjct: 658 GVLTSVHDANIGSIFGIGFPAWTGGAMQFIYGTGVEAFLKRADELA 703


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1052
Number of extensions: 44
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 727
Length adjustment: 39
Effective length of query: 660
Effective length of database: 688
Effective search space:   454080
Effective search space used:   454080
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources