Alignments for a candidate for gloB in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for gloB in Acidovorax sp. GW101-3H11

Align hydroxyacylglutathione hydrolase (EC 3.1.2.6) (characterized)
to candidate Ac3H11_478 Hydroxyacylglutathione hydrolase (EC 3.1.2.6)

Query= BRENDA::Q8ZRM2
         (251 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_478
          Length = 261

 Score =  206 bits (523), Expect = 5e-58
 Identities = 108/226 (47%), Positives = 147/226 (65%), Gaps = 10/226 (4%)

Query: 1   MNLNSIPAFQDNYIWVLTNDEGRCVIVDPGEAAPVLKAIAEHKWMPEAIFLTHHHHDHVG 60
           MNL  +PAF DNYIW+L +D  + ++VDPG+AAPV++A+       +AI +THHH DHVG
Sbjct: 1   MNLLPLPAFTDNYIWML-HDGRQAIVVDPGDAAPVVQALDTLGLGLQAILVTHHHADHVG 59

Query: 61  GVKELLQHFPQMTVYGPAETQDKGATHLVGDGDTIR---VLGEKFTLFATPGHTLGHVCY 117
           GV + L+      VYGPA  +       +  GD +    VLG +FT+   PGHT GH+ Y
Sbjct: 60  GV-DTLREATGARVYGPARERIPEPLVRLAQGDVVEGADVLGLRFTVIDVPGHTAGHIAY 118

Query: 118 FSR-----PYLFCGDTLFSGGCGRLFEGTPSQMYQSLMKINSLPDDTLICCAHEYTLANI 172
           +       P LFCGDTLFSGGCGRLFEGTP+QM  SL ++ +LP +T +CC HEYTL+N+
Sbjct: 119 YCEAMDGAPLLFCGDTLFSGGCGRLFEGTPAQMLDSLDQLAALPGNTRVCCTHEYTLSNL 178

Query: 173 KFALSILPHDSFINEYYRKVKELRVKKQMTLPVILKNERKINLFLR 218
           KFA ++ P ++ +  Y  + + LR ++Q TLP  +  ER IN FLR
Sbjct: 179 KFARAVEPGNAALLHYSSQCEALRAQQQPTLPSRMDLERDINPFLR 224


Lambda     K      H
   0.323    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 239
Number of extensions: 15
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 251
Length of database: 261
Length adjustment: 24
Effective length of query: 227
Effective length of database: 237
Effective search space:    53799
Effective search space used:    53799
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate Ac3H11_478 (Hydroxyacylglutathione hydrolase (EC 3.1.2.6))
to HMM TIGR03413 (gloB: hydroxyacylglutathione hydrolase (EC 3.1.2.6))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03413.hmm
# target sequence database:        /tmp/gapView.1264.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03413  [M=248]
Accession:   TIGR03413
Description: GSH_gloB: hydroxyacylglutathione hydrolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   2.8e-103  330.6   0.0   3.1e-103  330.4   0.0    1.0  1  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478  Hydroxyacylglutathione hydrolase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478  Hydroxyacylglutathione hydrolase (EC 3.1.2.6)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  330.4   0.0  3.1e-103  3.1e-103       3     248 .]       5     260 ..       3     260 .. 0.96

  Alignments for each domain:
  == domain 1  score: 330.4 bits;  conditional E-value: 3.1e-103
                                       TIGR03413   3 aipalsdNyiwllkdekseavvvDpgeaepvlealeekglkleaillTHhHaDHvggvaelle 65 
                                                     ++pa++dNyiw+l+d + +a+vvDpg+a+pv++al++ gl l+ail+THhHaDHvggv++l+e
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478   5 PLPAFTDNYIWMLHDGR-QAIVVDPGDAAPVVQALDTLGLGLQAILVTHHHADHVGGVDTLRE 66 
                                                     79**************9.********************************************* PP

                                       TIGR03413  66 kfpvkvvgpaeeripgltkevkegdevel...lelevevlevpGHtlgHiayyleee...kvl 122
                                                     +++++v+gpa+erip+  +++++gd ve    l+l+++v++vpGHt+gHiayy+e+    ++l
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478  67 ATGARVYGPARERIPEPLVRLAQGDVVEGadvLGLRFTVIDVPGHTAGHIAYYCEAMdgaPLL 129
                                                     *************************9975444999*******************9878889** PP

                                       TIGR03413 123 FcgDtLfsaGCGrlfegtaeqmleslqklaaLpeetkvycaHEYtlsNlrFalavepenealk 185
                                                     FcgDtLfs GCGrlfegt++qml+sl++laaLp +t+v+c+HEYtlsNl+Fa+avep n+al 
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478 130 FCGDTLFSGGCGRLFEGTPAQMLDSLDQLAALPGNTRVCCTHEYTLSNLKFARAVEPGNAALL 192
                                                     *************************************************************** PP

                                       TIGR03413 186 erlkevealrakgkptlPstlaeekatNpFLraeeaevkaalee.....ekaeevevfaelRe 243
                                                     ++ +++ealra+++ptlPs+++ e+++NpFLr+++++v++a++       ++++v+v+a+lR+
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478 193 HYSSQCEALRAQQQPTLPSRMDLERDINPFLRVRQPTVAQAARGhdaqvPENDAVAVLAALRQ 255
                                                     **************************************998877788888999********** PP

                                       TIGR03413 244 kkdkf 248
                                                     +k++f
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_478 256 WKNEF 260
                                                     ***98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (248 nodes)
Target sequences:                          1  (261 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 9.69
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources