GapMind for catabolism of small carbon sources

 

Alignments for a candidate for lacB in Acidovorax sp. GW101-3H11

Align predicted cytochrome c component of periplasmic glucoside 3-dehydrogenase (EC 1.1.99.13) (characterized)
to candidate Ac3H11_952 Cytochrome c551/c552

Query= reanno::Cola:Echvi_1841
         (141 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_952
          Length = 101

 Score = 86.3 bits (212), Expect = 1e-22
 Identities = 36/80 (45%), Positives = 56/80 (70%)

Query: 61  LALVKESDCPSCHMVERKIVGPAYKDVAEKYESTDENIETLAKRVVDGNNGVWGQVPMPA 120
           +AL    +C +CH V++K+VGP+YKDVA KY    + ++ LA ++V G +GVWG VPMPA
Sbjct: 22  MALATSKNCMACHAVDKKLVGPSYKDVAAKYAGQKDAVDKLAAKIVKGGSGVWGPVPMPA 81

Query: 121 HPGLSEDDAKKMVKYILMLK 140
           +  +++ DAKK+  ++L  K
Sbjct: 82  NAQVNDADAKKLAAWVLTQK 101


Lambda     K      H
   0.309    0.127    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 45
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 141
Length of database: 101
Length adjustment: 13
Effective length of query: 128
Effective length of database: 88
Effective search space:    11264
Effective search space used:    11264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 41 (20.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory