Alignments for a candidate for pccB in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccB in Acidovorax sp. GW101-3H11

Align propionyl-CoA carboxylase β subunit (EC 6.4.1.3) (characterized)
to candidate Ac3H11_4479 Biotin carboxylase (EC 6.3.4.14)

Query= metacyc::MONOMER-17284
         (514 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_4479
          Length = 1095

 Score =  212 bits (540), Expect = 5e-59
 Identities = 157/512 (30%), Positives = 241/512 (47%), Gaps = 32/512 (6%)

Query: 4    PEEQLATLFRLRER---ALQGGGPERVAQQHARGKLTARERLALLLDPNSFQEIGALASS 60
            P+   A L R+ +R         PE +A++HA+G  TARE +A L D  SF E GALA +
Sbjct: 579  PDHIRADLQRVIDRHAFTFDAARPEAIAKRHAQGGRTARENIADLCDNGSFIEYGALAIA 638

Query: 61   PLG------DEDQRVPGDGIVTGFGKINGR-------RVAVFAQDFTVMGGSFSAVQANK 107
                     D     P DG+VTG G ING        R  V + D TV+ G+  A    K
Sbjct: 639  AQARRRSKEDLIANTPADGMVTGIGGINGALFGDEKSRAVVMSYDATVLAGTQGARNHAK 698

Query: 108  ICRMQDLAIESGIPIIGLNDSGGARIQEGVRSLAA--YGEVFVRNVMASGVVPQISAILG 165
              RM  +A+   +P++   + GG R  +    + A  +   F      SG VP I    G
Sbjct: 699  TDRMLGIALAQKLPVVLFAEGGGGRPGDTDMPIVAGLHVHTFASYAALSGQVPVIGIAAG 758

Query: 166  PCAGGAVYSPALTDFVIMSESTGYMFLTGPDVIKAVSGRELSTQELGGAAVHCVRSGVAH 225
             C  G       +D +I +  +  + + GP +I+         +++G + V    +GV  
Sbjct: 759  RCFAGNAALLGCSDVIIATRGSN-IGMGGPAMIEGGGLGVFKPEQIGPSRVQHA-NGVID 816

Query: 226  LSAADDRAVIELIKRLLSYLPQNNNEDPPQIMPYDRADRIEPALNSLVPADERQGYDIHT 285
            +   D+   ++  +  LS+        P    P  R       L ++VP +  + YD  T
Sbjct: 817  VLVDDEAGAVQAARHYLSFF---QGRTPEWSAPDQRL------LRAVVPENRLRVYDTRT 867

Query: 286  LINLICDYDSFLEIQPLFAPNAVVGFARLDGYAVGIVANQPAVMAGALDIDASDKIARFV 345
             +  + D  S L ++  F        AR++G  VG++AN P  + GA+D DA+DK ARF+
Sbjct: 868  AMAGLVDDASLLPLRTGFGAGIHTALARIEGRPVGLLANNPQHLGGAIDADAADKGARFM 927

Query: 346  RICDAFNIPLITFVDTPGFLPGVEQEYGGVIRHGAKIIYAYSEATVPKISVVVRKAIGGA 405
            ++C+A  +P+++ VDTPGF+ G E E    +RH +++    +   VP  SVV+RK  G  
Sbjct: 928  QLCNAHGLPIVSLVDTPGFMVGPEVEATAQVRHVSRLFVTAASLRVPTFSVVLRKGYGLG 987

Query: 406  YVAMSSKQLRCDL-NFAWPSAQIAVMGAEGAVRILRREELRTAENPAQLMEQFIADYRRR 464
             + M++      +   AWP+ +   MG EGAVR+  R+EL      A+    F     + 
Sbjct: 988  AMGMTAGGFHAPVFTVAWPTGEFGAMGLEGAVRLGFRKELEALPEGAERDALFAKLLAKS 1047

Query: 465  YFN--PYHAADLGQIDEVIEPAETRPRLIRAL 494
            Y N    H A   +ID VI+PA+TR  L R L
Sbjct: 1048 YANGEAMHMAATLEIDAVIDPADTRAWLARGL 1079


Lambda     K      H
   0.321    0.138    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1402
Number of extensions: 64
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 514
Length of database: 1095
Length adjustment: 40
Effective length of query: 474
Effective length of database: 1055
Effective search space:   500070
Effective search space used:   500070
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources