Aligments for a candidate for gtsA in Acidovorax sp. GW101-3H11

GapMind for catabolism of small carbon sources

Aligments for a candidate for gtsA in Acidovorax sp. GW101-3H11

Align ABC transporter for D-Glucose-6-Phosphate, periplasmic substrate-binding component (characterized)
to candidate Ac3H11_2062 ABC-type sugar transport system, periplasmic component

Query= reanno::WCS417:GFF4324
         (428 letters)



>lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2062 ABC-type sugar
           transport system, periplasmic component
          Length = 416

 Score =  430 bits (1105), Expect = e-125
 Identities = 222/419 (52%), Positives = 277/419 (66%), Gaps = 12/419 (2%)

Query: 8   AVAISIASLFPLSAFAADSKGTVEVVHWWTSGGEKAAVDVLKAQVEKDGFVWKDGAVAGG 67
           AVA+ +A+    SA      G VEV+H+WTSGGE  +V  LK  ++  G  W+D AVAGG
Sbjct: 9   AVAVGLAAAMSASA------GEVEVLHYWTSGGEAKSVAELKKIMQGKGHTWRDFAVAGG 62

Query: 68  GGATAMTVLKSRAVAGNPPGVAQIKGPDIQEWASTGLLDTDVLKDVAKEEKWDSLLDKKV 127
           GG +AMTVLKSR ++GNPP  AQ KGP IQEWAS G+L    +  +AK EKWD LL K V
Sbjct: 63  GGDSAMTVLKSRVISGNPPSAAQTKGPAIQEWASEGVLAN--MDTLAKAEKWDELLPKVV 120

Query: 128 SDTVKYEGDYVAVPVNIHRVNWLWINPEVFKKAGITKNPTTLQEFYAAGDKLKAAGFIPL 187
           +D +KY+G YVA PVN+HRVNW+W + E  KKAG+   P T  EF+AA DKLKAAG +P+
Sbjct: 121 ADVMKYKGAYVAAPVNVHRVNWMWGSSEALKKAGVAAMPKTWDEFFAAADKLKAAGLVPV 180

Query: 188 AHGGQPWQDSTVFEAVVLSVMGADGYKKALVDLDNGALTGPEMVKALTELKKVATYMDVD 247
           AHGGQ WQD T FE+VVL V GA  Y+ ALV LDN ALT   M K+L   +++  Y D  
Sbjct: 181 AHGGQNWQDFTTFESVVLGVGGAKFYQDALVKLDNTALTSDTMKKSLETFRRIKGYTDPG 240

Query: 248 GKGQDWNLEAGKVINGKAGMQIMGDWAKSEWTAAKKVAGKDYECVAFPGTDKAFTYNIDS 307
             G+DWNL    +I GKAG Q+MGDWAK E+ AA K  GKD+ C A PG+  AFT+N+DS
Sbjct: 241 APGRDWNLATAMLIQGKAGFQLMGDWAKGEFLAAGKAPGKDFLCAAAPGSANAFTFNVDS 300

Query: 308 LAVFKQKDKGTAAGQQDIAKVVLGENFQKVFSINKGSIPVRNDMLNKMDSYGFDSCAQTA 367
             +FK KD      Q D+A  ++   FQ+VF++NKGSIPVR      MD   FD CA+ +
Sbjct: 301 FILFKLKDAAAQKAQSDLASSIMSPAFQEVFNLNKGSIPVRAG--QPMDK--FDDCAKAS 356

Query: 368 AKDFLADAKTGGLQPSMAHNMATTLAVQGAFFDVVTNYINDPKADPADTAKKLGAAIKS 426
           AKDF+  AK+GGL PS AH MA   A +GA  DVV+ + ND K   AD  KK+ AA K+
Sbjct: 357 AKDFVDTAKSGGLVPSAAHGMAIAPATEGAIKDVVSQFWNDDKVSVADAMKKIAAAAKT 415


Lambda     K      H
   0.314    0.131    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 583
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 428
Length of database: 416
Length adjustment: 32
Effective length of query: 396
Effective length of database: 384
Effective search space:   152064
Effective search space used:   152064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources