GapMind for catabolism of small carbon sources

 

Protein AZOBR_RS08235 in Azospirillum brasilense Sp245

Annotation: FitnessBrowser__azobra:AZOBR_RS08235

Length: 301 amino acids

Source: azobra in FitnessBrowser

Candidate for 15 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-alanine catabolism AZOBR_RS08235 hi L-proline and D-alanine ABC transporter, permease component 1 (characterized) 100% 100% 580.1 High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine 57% 351.7
L-proline catabolism AZOBR_RS08235 hi L-proline and D-alanine ABC transporter, permease component 1 (characterized) 100% 100% 580.1 High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine 57% 351.7
L-arginine catabolism braD med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-glutamate catabolism braD med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-histidine catabolism braD med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-isoleucine catabolism livH med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-leucine catabolism livH med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-valine catabolism livH med Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) 64% 100% 380.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-alanine catabolism braD med High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 57% 98% 351.7 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-phenylalanine catabolism livH med Branched-chain amino acid ABC transporter permease LivH; SubName: Full=Branched-chain amino acid transporter permease subunit LivH; SubName: Full=L-leucine ABC transporter membrane protein /L-isoleucine ABC transporter membrane protein /L-valine ABC transporter membrane protein (characterized, see rationale) 57% 98% 351.7 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-serine catabolism braD med High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 57% 98% 351.7 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-threonine catabolism braD med High-affinity branched-chain amino acid transport system permease protein BraD, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 57% 98% 351.7 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-proline catabolism HSERO_RS00885 med ABC transporter permease (characterized, see rationale) 46% 98% 261.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-serine catabolism Ac3H11_1695 med ABC transporter permease (characterized, see rationale) 46% 98% 261.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1
L-tyrosine catabolism Ac3H11_1695 med ABC transporter permease (characterized, see rationale) 46% 98% 261.2 L-proline and D-alanine ABC transporter, permease component 1 100% 580.1

Sequence Analysis Tools

View AZOBR_RS08235 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

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Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Sequence

MEYFLQQLINGLSLGAIYGLIAIGYTMVYGIIGMINFAHGEIYMIGAFVALITFLAIGSL
GITWVPLALLVMLVASMLFTAVYGWTVERIAYRPLRSSPRLAPLISAIGMSIFLQNYVQI
LQGARSKPLQPILPGNLTLMDGAVSVSYVRLATIVITIALMYGFTQLITRTSLGRAQRAC
EQDKKMAGLLGVNVDRVISLTFVMGAALAAVAGMMVLLIYGVIDFYIGFLAGVKAFTAAV
LGGIGSLPGAMLGGVVIGLIEAFWSGYMGSEWKDVATFTILVLVLIFRPTGLLGRPEIEK
V

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory