Protein AZOBR_RS08250 in Azospirillum brasilense Sp245
Annotation: FitnessBrowser__azobra:AZOBR_RS08250
Length: 236 amino acids
Source: azobra in FitnessBrowser
Candidate for 19 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-alanine catabolism | AZOBR_RS08250 | hi | Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) | 100% | 100% | 457.2 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-proline catabolism | AZOBR_RS08250 | hi | Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) | 100% | 100% | 457.2 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-arginine catabolism | braG | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-glutamate catabolism | braG | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-histidine catabolism | braG | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-isoleucine catabolism | livF | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW | 48% | 208.0 |
| L-leucine catabolism | livF | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW | 48% | 208.0 |
| L-valine catabolism | livF | hi | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 70% | 96% | 337.8 | Branched-chain amino acid transport system ATP-binding protein, component of The phenylpropeneoid uptake porter, CouPSTW | 48% | 208.0 |
| L-phenylalanine catabolism | livF | hi | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF (characterized) | 62% | 98% | 270 | ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM | 53% | 243.4 |
| L-alanine catabolism | braG | med | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) | 59% | 100% | 273.1 | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF | 62% | 270.0 |
| L-serine catabolism | braG | med | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) | 59% | 100% | 273.1 | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF | 62% | 270.0 |
| L-threonine catabolism | braG | med | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) | 59% | 100% | 273.1 | high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF | 62% | 270.0 |
| L-proline catabolism | HSERO_RS00900 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 51% | 96% | 233 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-serine catabolism | Ac3H11_1692 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 51% | 96% | 233 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-tyrosine catabolism | Ac3H11_1692 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 51% | 96% | 233 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-isoleucine catabolism | natE | med | NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 46% | 95% | 205.3 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-leucine catabolism | natE | med | NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 46% | 95% | 205.3 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-proline catabolism | natE | med | NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 46% | 95% | 205.3 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
| L-valine catabolism | natE | med | NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 46% | 95% | 205.3 | High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine | 59% | 273.1 |
Sequence Analysis Tools
View AZOBR_RS08250 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
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Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MLKVSGVHTFYGAIEALKGVDIEIGAGEIVSLIGANGAGKSTLLMTICGSPRARMGRITF
EGQDITQMPTYELVRLGIAQSPEGRRIFPRMSVLENLQMGSITAKPGSFANELERVLTLF
PRLKERISQRAGTMSGGEQQMLAIGRALMSQPRLLLLDEPSLGLAPLVVKQIFQAVKDIN
REQKMTVFMVEQNAFHALKLAHRGYVMVNGKVTMSGTGAELLANEEVRSAYLEGGH
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory