Protein AZOBR_RS15680 in Azospirillum brasilense Sp245
Annotation: FitnessBrowser__azobra:AZOBR_RS15680
Length: 216 amino acids
Source: azobra in FitnessBrowser
Candidate for 19 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-asparagine catabolism | natH | lo | Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (characterized, see rationale) | 37% | 54% | 133.3 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-aspartate catabolism | natH | lo | Amino acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine (characterized, see rationale) | 37% | 54% | 133.3 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| D-alanine catabolism | Pf6N2E2_5404 | lo | ABC transporter for D-Alanine, permease component 1 (characterized) | 34% | 59% | 122.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-lysine catabolism | hisM | lo | ABC transporter for L-Lysine, permease component 2 (characterized) | 35% | 90% | 122.1 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-lysine catabolism | hisQ | lo | ABC transporter for L-Lysine, permease component 1 (characterized) | 33% | 94% | 119 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-histidine catabolism | hisM | lo | Histidine transport system permease protein HisM (characterized) | 35% | 84% | 114.4 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-glutamate catabolism | gltJ | lo | Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) | 33% | 87% | 112.1 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-histidine catabolism | aapM | lo | ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 2 (characterized) | 32% | 54% | 112.1 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-asparagine catabolism | aatQ | lo | ABC transporter for L-asparagine and L-glutamate, permease component 1 (characterized) | 34% | 88% | 109.4 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-aspartate catabolism | aatQ | lo | ABC transporter for L-asparagine and L-glutamate, permease component 1 (characterized) | 34% | 88% | 109.4 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-citrulline catabolism | AO353_03045 | lo | ABC transporter for L-Arginine and L-Citrulline, permease component 1 (characterized) | 32% | 98% | 107.5 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-asparagine catabolism | aapM | lo | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 30% | 54% | 105.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-aspartate catabolism | aapM | lo | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 30% | 54% | 105.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-glutamate catabolism | aapM | lo | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 30% | 54% | 105.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-leucine catabolism | aapM | lo | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 30% | 54% | 105.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-proline catabolism | aapM | lo | AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) | 30% | 54% | 105.9 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-glutamate catabolism | gluC | lo | GluC aka CGL1952, component of Glutamate porter (characterized) | 35% | 94% | 104.4 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| L-histidine catabolism | BPHYT_RS24005 | lo | Polar amino acid ABC transporter, inner membrane subunit; Flags: Precursor (characterized, see rationale) | 30% | 82% | 100.1 | L-cystine transport system permease protein YecS | 39% | 151.0 |
| D-alanine catabolism | Pf6N2E2_5403 | lo | ABC transporter for D-Alanine, permease component 2 (characterized) | 33% | 56% | 84 | L-cystine transport system permease protein YecS | 39% | 151.0 |
Sequence Analysis Tools
View AZOBR_RS15680 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
IRAFSYNEFLFLLSAVQWTLALSAIAFIGGATVGLVIALARTSDIKAVRLAATGYIQLFQ
GTPLLMQLFLVFFGATVMGLDLNPWAAAALGLTLNASAFLGEIWRGCIQAVPRGQWEAAS
ALGLRYPGLMRYVILPQAVKVAVPPTVGFLVQLIKSTSLAAIIGFTELTRAGQIVNNATF
QPFLVFGIVAVLYFLMCWPLSLLSARLERRYALATR
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory