GapMind for catabolism of small carbon sources

 

Protein AZOBR_RS15915 in Azospirillum brasilense Sp245

Annotation: FitnessBrowser__azobra:AZOBR_RS15915

Length: 337 amino acids

Source: azobra in FitnessBrowser

Candidate for 5 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-gluconate catabolism gntC hi TRAP dicarboxylate transport system, periplasmic component (DctP-like) (characterized, see rationale) 100% 100% 656.4 Putative TRAP transporter solute receptor DctP, component of The possible disulfide 3,3'-dithiodipropionic acid (DTDP) tripartite transporter, DctMPQ 50% 338.6
succinate catabolism dctP lo TRAP dicarboxylate transporter DctP subunit; Flags: Precursor (characterized, see rationale) 33% 97% 180.3 D-gluconate TRAP transporter, periplasmic component 63% 417.2
L-malate catabolism dctP lo C4-dicarboxylate-binding periplasmic protein DctP (characterized) 31% 96% 178.3 D-gluconate TRAP transporter, periplasmic component 63% 417.2
fumarate catabolism dctP lo C4-dicarboxylate-binding periplasmic protein DctP (characterized) 31% 96% 178.3 D-gluconate TRAP transporter, periplasmic component 63% 417.2
D-glucuronate catabolism dctP lo Solute-binding protein Bamb_6123 (characterized) 31% 92% 146.4 D-gluconate TRAP transporter, periplasmic component 63% 417.2

Sequence Analysis Tools

View AZOBR_RS15915 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MKLLRSVLLATGLAAAILAPVAASAQDIKPRLIRFGYGLSESSNQGRAVKFFVEDMAKRS
GGKLKVKGFADASLGSDIQMQNALIGGAQEMMVGSTATLVGIVKDFAVFDLPFLFNNEQE
ADAVFDGPFGQKLAAKLNDKGLVGLVYWENGFRNLTNSKRPVEKVEDLKGIKLRVMQNPV
YIDMFNGFGANAVPLSFSELFTAMETGTVDGQENPVTTIQSSKFYEVQKYLTISKHVYSP
WIVLASKRWYDGLSADERKIINEAAVASRDFERKDSREASKQSIAYLKDKGMQINELSDA
ELGRMREMVKPAMDKFAADGGADLLNELQGEISKVRK

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory