Align BadI (characterized)
to candidate AZOBR_RS26485 AZOBR_RS26485 enoyl-CoA hydratase
Query= metacyc::MONOMER-892 (260 letters) >FitnessBrowser__azobra:AZOBR_RS26485 Length = 262 Score = 111 bits (277), Expect = 2e-29 Identities = 85/260 (32%), Positives = 121/260 (46%), Gaps = 6/260 (2%) Query: 1 MQFEDLIYEIRNG-VAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAGD 59 M E I R+G V I INRP +NA T EL +AL + D V IV+ GAG+ Sbjct: 1 MTDERPILTSRDGAVGLITINRPTTLNALDVPTLLELERALGELESDDGVHVIVVTGAGE 60 Query: 60 RAFCTGGDQSTHDGNYDGRGTVGLPMEELHTAIR---DVPKPVIARVQGYAIGGGNVLAT 116 RAF GGD + D + E +H R KP IA V G+A+GGG L Sbjct: 61 RAFVAGGDIADLDSRQGLAHYLDFA-EVVHRVFRRFETCDKPTIAAVNGWALGGGTELVL 119 Query: 117 ICDLTICSEKAIFGQVGPKMGSVDPGYGTAFLARVVGEKKAREIWYMCKRYSGKEAEAMG 176 D+ I ++ A FG +G GT + R + +ARE+ + +++ EA A G Sbjct: 120 SLDIRIAADTAKFGLPEINLGLFPGAGGTQRILRQIPPCRARELVFTGDQFTAAEAVAWG 179 Query: 177 LANLCVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIA-GMGMYALKLYY 235 L N VP +L AE +++ +SP L + KR+ G + + L Sbjct: 180 LVNRAVPKADLMAEAMATAQKIAAKSPLILKLTKRTLRHGAEMPLGASLAYEQAMIGLVL 239 Query: 236 DTDESREGVKALQEKRKPEF 255 D+ ++ EG +A EKRK F Sbjct: 240 DSQDAHEGCRAFLEKRKAAF 259 Lambda K H 0.319 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 180 Number of extensions: 10 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 262 Length adjustment: 25 Effective length of query: 235 Effective length of database: 237 Effective search space: 55695 Effective search space used: 55695 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory