Align Leucine//isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale)
to candidate AZOBR_RS32400 AZOBR_RS32400 leucine/isoleucine/valine transporter ATP-binding subunit
Query= uniprot:G8ALJ1 (236 letters) >FitnessBrowser__azobra:AZOBR_RS32400 Length = 232 Score = 226 bits (577), Expect = 2e-64 Identities = 121/234 (51%), Positives = 168/234 (71%), Gaps = 2/234 (0%) Query: 1 MLKVSGVHTFYGAIEALKGVDIEIGAGEIVSLIGANGAGKSTLLMTICGSPRARMGRITF 60 +L+V + G E L+GV +++ G IV+++GANG GK+TL+ + G R G F Sbjct: 1 ILEVRDLTVRMGPQEILRGVSLDVAEGAIVAVLGANGVGKTTLMRALSGIYRTSGGATVF 60 Query: 61 EGQDITQMPTYELVRLGIAQSPEGRRIFPRMSVLENLQMGSITAKPGSFANELERVLTLF 120 +G+D+T P++E+VR G+AQ+PEGR+IF M+V ENL +G T + A +ER+L LF Sbjct: 61 DGEDMTDSPSHEVVRRGLAQAPEGRQIFGTMTVRENLLLGGRTLRHDRAAR-MERMLELF 119 Query: 121 PRLKERISQRAGTMSGGEQQMLAIGRALMSQPRLLLLDEPSLGLAPLVVKQIFQAVKDIN 180 PRL+ER+ Q+AG++SGGEQQML I RALMS+PRLLLLDEPSLGLAP+VVKQIF + I Sbjct: 120 PRLRERLIQKAGSLSGGEQQMLCIARALMSKPRLLLLDEPSLGLAPMVVKQIFDLLVQI- 178 Query: 181 REQKMTVFMVEQNAFHALKLAHRGYVMVNGKVTMSGTGAELLANEEVRSAYLEG 234 R + ++ +VEQNA AL++A YVM G+VT+SG+ A+L A+ VR+AYL G Sbjct: 179 RGEGTSILLVEQNARAALRVADHAYVMEAGRVTLSGSAADLAADPRVRAAYLGG 232 Lambda K H 0.320 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 193 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 236 Length of database: 232 Length adjustment: 23 Effective length of query: 213 Effective length of database: 209 Effective search space: 44517 Effective search space used: 44517 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory