Align D-lactate transporter, permease component 2 (characterized)
to candidate AZOBR_RS11650 AZOBR_RS11650 ABC transporter permease
Query= reanno::Phaeo:GFF1250 (340 letters) >FitnessBrowser__azobra:AZOBR_RS11650 Length = 613 Score = 167 bits (424), Expect = 5e-46 Identities = 104/334 (31%), Positives = 173/334 (51%), Gaps = 46/334 (13%) Query: 1 MDAILLQILNGLDKGSAYALIALGLTLIFGTLGVVNFAHGALFMIGAFCAVTVQRVLSLS 60 M L+Q L+GL + L++ GLT++FG +VNFAHG+ +M+GA+ Sbjct: 1 MSFYLVQFLSGLATAATLFLVSSGLTIVFGVTRIVNFAHGSFYMLGAY------------ 48 Query: 61 FETVDETQKDFLGNPLKVKTPYVESWFGPEVGGAIIDWAVPLAILFAIPIMIGVGYVMER 120 LG L VE W +G W A A+ ++ G +ME Sbjct: 49 -----------LGWML------VERWGSTPLGF----WGAVAAASLAVGLL---GALMEV 84 Query: 121 GLIKHFYKRPHADQILVTFGLAIVLQEVVKYFYGANPIQTPAPDALNGVVNLGSIIGMDI 180 GL++ Y+ P Q+L TFG+ +V+Q+ + +G+ + L G V++ +D Sbjct: 85 GLLRRLYRSPELFQLLATFGVVLVVQDAASWIWGSEDLLGRRAPGLRGSVDI-----LDQ 139 Query: 181 VYPVWRVVYFFFAVVIIGGIFSFLQFTTFGMVVRAGMADRETVGLLGINIDRRFTIMFGI 240 +P++ +V +++G ++ T +G +VRA DR+ LG++ R FT + + Sbjct: 140 PFPLYDLVLIGLGPLVLGLLWLLFNRTRWGTLVRAATQDRDMASALGVDPARLFTGVLFL 199 Query: 241 AAAVAGLAGVMYTPINSPNYHMGMDFLVLSFVVVVVGGMGSLPGAVLAGFLLGVLESFAS 300 +A+AGL G + P + N HM M +V +FVVVVVGGMGSL GA +A L+G L++F Sbjct: 200 GSALAGLGGALQVPREAVNLHMDMAIVVEAFVVVVVGGMGSLTGAFVASLLIGQLQAFGI 259 Query: 301 MNEIKSLIPGIDQIIIYVVAIIILLTRPRGLMGR 334 + + P I +++++ ++L+ RP GL+GR Sbjct: 260 L-----VFPQITLVLVFLFMAVVLVIRPYGLLGR 288 Score = 63.2 bits (152), Expect = 2e-14 Identities = 76/324 (23%), Positives = 134/324 (41%), Gaps = 74/324 (22%) Query: 19 ALIALGLTLIFGTLGVVNFAHGALFMIGAFCAVTVQRVLSLSFETVDETQKDFLGNPLKV 78 AL+A L L+ G G+V+F H A F +GA+ A + Sbjct: 343 ALLAASLHLLIGLGGLVSFGHAAYFGLGAYGAALL------------------------- 377 Query: 79 KTPYVESWFGPEVGGAIIDWAVPLAILFAIPIMIGVGYVMERGLIKHFYKRPHADQILVT 138 V+ + P +PLA L A P++ G+G ++ +R ++T Sbjct: 378 ----VKHFAAP----------MPLA-LAAAPLLAGLGALVAGWFC---VRRSGLYFAMLT 419 Query: 139 FGLAIVLQEVVKYFYGANPIQTPAPDALNGVVNLGSIIGMDIVYPVW---RVVYFFFAVV 195 A ++ V +YG NG++ + P W + Y++ + Sbjct: 420 LAFAQIVWSVTFQWYGFT-------GGDNGILGVWP--------PDWAAGKAAYYWLTLA 464 Query: 196 IIGGIFSFLQ---FTTFGMVVRAGMADRETVGLLGINIDRRFTIMFGIAAAVAGLAGVMY 252 + GG L+ F FG +RAG +GI++ R + F +A + AGLAG +Y Sbjct: 465 MAGGALLLLRRAAFAPFGYTLRAGRDSALRAESVGIDVRRHQWLSFALAGSAAGLAGGLY 524 Query: 253 TPINSPNYH--MGMDFLVLSFVVVVVGGMGSLPGAVLAGFLLGVLESFA-SMNEIKSLIP 309 + M + V + V+V++GG+ +L G V+ L +LE+ A S + L+ Sbjct: 525 AFSKGSVFPTLMAVPVSVDALVMVLMGGIQTLSGPVVGAALFHLLETEAMSRTDWWRLVL 584 Query: 310 GIDQIIIYVVAIIILLTRPRGLMG 333 G + ++++L P+G+ G Sbjct: 585 G-------TIILVLVLAFPKGVAG 601 Lambda K H 0.329 0.147 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 508 Number of extensions: 29 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 4 Number of HSP's successfully gapped: 4 Length of query: 340 Length of database: 613 Length adjustment: 33 Effective length of query: 307 Effective length of database: 580 Effective search space: 178060 Effective search space used: 178060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory