Align L-lactate dehydrogenase (EC 1.1.1.27) (characterized)
to candidate AZOBR_RS14060 AZOBR_RS14060 malate dehydrogenase
Query= BRENDA::Q8I8U5 (330 letters) >FitnessBrowser__azobra:AZOBR_RS14060 Length = 322 Score = 343 bits (879), Expect = 4e-99 Identities = 176/322 (54%), Positives = 229/322 (71%), Gaps = 5/322 (1%) Query: 9 RPKIAMVGSGMIGGTMAFLCSLRELGDVVLFDVVPNMPMGKAMDISHNSSVVDTGITVYG 68 R KIA+VG+G IGGT+A L + +ELGDVVLFD+ MP GKA+D++ S V + G Sbjct: 3 RKKIALVGAGQIGGTLALLAAQKELGDVVLFDIAEGMPEGKALDLAETSPVEGFNAKLSG 62 Query: 69 SNSYECLKGADVVIITAGITKIPGKSDKEWSRMDLLPVNIKIMREVGAAIKSYCPNAFVI 128 N Y ++GADVVI+TAG+ + PG S R DL+ +N + + VG IK Y PNAFVI Sbjct: 63 GNDYSVIEGADVVIVTAGVPRKPGMS-----RDDLIGINTGVCQTVGENIKKYAPNAFVI 117 Query: 129 NITNPLDVMVAALQESSGLPHHRICGMAGMLDSSRFRRMIADKLEVSPRDVQGMVIGVHG 188 ITNPLDVMV LQ++SGLP R+ GMAG+LDS+RFR +AD+ +VS DV V+G HG Sbjct: 118 VITNPLDVMVWVLQQASGLPPERVVGMAGVLDSARFRYFLADEFKVSVEDVTAFVLGGHG 177 Query: 189 DHMVPLSRYATVNGIPLSEFVKKGWIKQEEVDDIVQKTKVAGGEIVRLLGQGSAYYAPGA 248 D MVPL RY+TV GIPL + VK GW QE++D IVQ+T+ G EIV+LL GSA+YAP A Sbjct: 178 DTMVPLVRYSTVAGIPLPDLVKMGWTTQEKLDAIVQRTRDGGAEIVKLLKTGSAFYAPAA 237 Query: 249 SAIQMAESYLKDRKRVMVCSCYLQGQYGVQNHYLGVPCVIGGRGVEKIIELELTAQERQE 308 SAIQMAESYLKD+KRV+ + YL GQYG + Y+GVP +IG GVEKIIE++L+A+E++ Sbjct: 238 SAIQMAESYLKDQKRVVPVAAYLTGQYGQNDLYVGVPTIIGAGGVEKIIEIDLSAEEKKM 297 Query: 309 LQGSIDEVKEMQKAIAALDASK 330 S+D VK++ + + A K Sbjct: 298 FDHSVDAVKQLVEVVKKQQAEK 319 Lambda K H 0.319 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 339 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 330 Length of database: 322 Length adjustment: 28 Effective length of query: 302 Effective length of database: 294 Effective search space: 88788 Effective search space used: 88788 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory